Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCU50_RS13085 Genome accession   NZ_AP025514
Coordinates   2870794..2871405 (-) Length   203 a.a.
NCBI ID   WP_060468742.1    Uniprot ID   A0A120DG30
Organism   Vibrio toranzoniae strain CECT 7225     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2865794..2876405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU50_RS13065 aroB 2866729..2867817 (-) 1089 WP_060468739.1 3-dehydroquinate synthase -
  OCU50_RS13070 aroK 2867843..2868361 (-) 519 WP_017061125.1 shikimate kinase AroK -
  OCU50_RS13075 pilQ 2868538..2870229 (-) 1692 WP_060468740.1 type IV pilus secretin PilQ Machinery gene
  OCU50_RS13080 pilP 2870289..2870807 (-) 519 WP_060468741.1 pilus assembly protein PilP Machinery gene
  OCU50_RS13085 pilO 2870794..2871405 (-) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU50_RS13090 pilN 2871398..2871997 (-) 600 WP_060468743.1 PilN domain-containing protein Machinery gene
  OCU50_RS13095 pilM 2871981..2873006 (-) 1026 WP_060468744.1 type IV pilus assembly protein PilM Machinery gene
  OCU50_RS13100 - 2873158..2875719 (+) 2562 WP_060468745.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23477.94 Da        Isoelectric Point: 4.7445

>NTDB_id=92421 OCU50_RS13085 WP_060468742.1 2870794..2871405(-) (pilO) [Vibrio toranzoniae strain CECT 7225]
MVSFQNRVSLQDLDVDEITEWPLLPQLLVIFALMILIQGVGTWLYIAPLDDELQQMKQQEQTLKTTLRIKANKAAVLPKL
QSQLDDLTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHAIGDFSAAIA
KLPRIISFNDVSWQRVSQESSTLHFRVRAYTYQFKPELSDETQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=92421 OCU50_RS13085 WP_060468742.1 2870794..2871405(-) (pilO) [Vibrio toranzoniae strain CECT 7225]
ATGGTTAGTTTTCAAAATCGGGTGAGCTTGCAAGATCTCGATGTTGATGAGATCACCGAATGGCCTTTATTGCCTCAGCT
ATTGGTCATTTTTGCCTTGATGATACTTATCCAAGGTGTGGGGACTTGGTTGTATATTGCTCCTTTGGACGATGAACTTC
AGCAGATGAAACAGCAAGAACAGACGCTTAAAACAACTTTGCGGATTAAAGCCAATAAGGCGGCGGTTTTGCCTAAGCTC
CAGAGTCAGTTGGATGATTTAACCAGTCGTTATGATTATCTATTAGAGCAGCTACCAGTACAAAAAGAGTTGGCAAGTAT
GTTGGCCTCAGTGAATGAGCTTGGGTTGGATAATAAGCTGACGTTTACGCGTATTGATTGGGGACAGAAGCAAAACAAGG
AGTTTCTCTATCGTTTACCGCTCAATATAGAGCTAACAGGGGACTATCACGCGATTGGAGACTTCTCAGCAGCCATCGCC
AAACTCCCACGAATCATTAGTTTTAATGATGTGAGTTGGCAGCGCGTCAGCCAAGAAAGTAGCACGCTCCATTTCAGGGT
GCGAGCGTATACCTACCAATTTAAGCCGGAGTTGAGTGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A120DG30

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

73.711

95.567

0.704
  pilO Vibrio campbellii strain DS40M4

70.37

93.103

0.655

Multiple sequence alignment