Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OCU50_RS13075 Genome accession   NZ_AP025514
Coordinates   2868538..2870229 (-) Length   563 a.a.
NCBI ID   WP_060468740.1    Uniprot ID   -
Organism   Vibrio toranzoniae strain CECT 7225     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2863538..2875229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU50_RS13055 - 2864217..2865062 (-) 846 WP_060468737.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  OCU50_RS13060 - 2865132..2866649 (-) 1518 WP_060468738.1 SPOR domain-containing protein -
  OCU50_RS13065 aroB 2866729..2867817 (-) 1089 WP_060468739.1 3-dehydroquinate synthase -
  OCU50_RS13070 aroK 2867843..2868361 (-) 519 WP_017061125.1 shikimate kinase AroK -
  OCU50_RS13075 pilQ 2868538..2870229 (-) 1692 WP_060468740.1 type IV pilus secretin PilQ Machinery gene
  OCU50_RS13080 pilP 2870289..2870807 (-) 519 WP_060468741.1 pilus assembly protein PilP Machinery gene
  OCU50_RS13085 pilO 2870794..2871405 (-) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU50_RS13090 pilN 2871398..2871997 (-) 600 WP_060468743.1 PilN domain-containing protein Machinery gene
  OCU50_RS13095 pilM 2871981..2873006 (-) 1026 WP_060468744.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61329.92 Da        Isoelectric Point: 4.4300

>NTDB_id=92419 OCU50_RS13075 WP_060468740.1 2868538..2870229(-) (pilQ) [Vibrio toranzoniae strain CECT 7225]
MNKGLQGLIVSVMLIFSVLSHAEDLPNKLENIDFRVNKNKDAVLIIELATSTVVVDVQRAQEGLSIELINTHVDDNKLQL
LDVKDFATLVEGVEVYKEAPSTRLLATISKDYQYEYNLKGRFIEVVISKPAIEEAVTEKSILEKEGKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREQQALEKSRLEEELG
ELKSEIIKIHFAKATDIADMIGGDGTVSMLSDRGSITIDERTNSLLIRELEENIAVIRSIIESLDIPVKQVQIEARIVTV
TEGNLDELGVRWGASSTNGSFTVGGSIEGNYPSIAPYDSNGGNSVIDEYLNVNLGATSSNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=92419 OCU50_RS13075 WP_060468740.1 2868538..2870229(-) (pilQ) [Vibrio toranzoniae strain CECT 7225]
ATGAACAAAGGGTTACAAGGCTTGATTGTGTCTGTGATGTTGATCTTTAGTGTGCTCAGTCACGCTGAGGACTTACCTAA
TAAGTTAGAGAATATTGATTTTAGGGTGAACAAAAATAAAGACGCTGTCCTAATTATCGAACTCGCAACCAGCACGGTTG
TGGTGGATGTTCAACGTGCGCAAGAGGGGCTAAGCATAGAGCTGATCAATACTCATGTTGATGACAACAAACTCCAGCTT
CTTGATGTGAAAGATTTCGCTACCTTGGTTGAAGGCGTGGAAGTGTATAAAGAGGCCCCAAGCACTCGGTTGTTAGCGAC
AATCAGCAAGGACTACCAATACGAATACAATCTCAAAGGCCGTTTTATTGAGGTGGTGATCAGTAAGCCTGCTATAGAAG
AAGCGGTAACAGAAAAAAGTATACTCGAGAAAGAGGGTAAGCTGATATCGATTAACTTCCAAGATATTCCAGTGCGAAAT
GTCCTGCAGCTTATCGCTGACTATAACGACTTTAACTTAGTCGTGTCTGATTCAGTAACCGGAAACCTAACTTTACGTTT
AGATGGTGTGCCTTGGCAGCAAGTTCTGGATATCATCTTACAGGTCAAAGGGTTAGATAAGCGGGTAGATGGCAATGTCA
TCTTGGTAGCGCCAAAAGATGAACTCGACCTTCGAGAGCAGCAAGCGTTAGAGAAGTCGCGTTTAGAAGAGGAATTGGGT
GAGCTTAAGTCGGAAATTATTAAGATTCACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGTGGTGACGGGACGGT
GAGCATGTTGTCCGATCGTGGCTCGATTACTATTGATGAGCGCACCAATTCATTGCTTATTCGAGAACTGGAGGAAAACA
TTGCCGTGATACGAAGCATTATTGAATCTTTAGATATTCCTGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTGACTGTT
ACTGAGGGGAACCTTGATGAGCTAGGAGTGCGTTGGGGAGCATCTTCAACAAACGGAAGCTTCACGGTTGGTGGTTCTAT
CGAAGGCAACTACCCGTCGATTGCACCTTACGATAGTAATGGGGGGAATAGCGTCATTGATGAGTACCTCAACGTTAACT
TAGGTGCGACATCTTCAAATGCATCAAGCATTGCCTTTCAGGTTGCCAAACTGGGCTCTGATACCTTGCTCGACCTTGAG
TTATCAGCGCTACAGCAAGAGTCAAAAGCTGAAATTATTTCGAGTCCGCGTTTGATTACCACCAATAAAAAGCCCGCTTA
TATTGAACAAGGTACTGAAATTCCTTATTTAGAGTCATCTTCAAGTGGTGCAACGTCAGTCGCATTTAAGAAAGCGGTAT
TGAGCCTTAAGGTGACTCCACAGATCACGCCTGACAATCGTTTAGTGTTGGATTTGAGCGTAACTCAAGATAGGCCCGGA
CAGGTGGTGAAAACCGGAACTGGAGAGGCTGTTGCAATTGATACTCAGCGCATTGGCACGCAAGTGCTTGTTAATAATGG
TGAAACGGTCGTTCTTGGTGGGATATTTCAGCACAGTGTGAGTAGCACCGTCGATAAAGTTCCTCTATTGGGAGACCTGC
CTGTATTAGGGGCTTTGTTCCGCAGAAGCTACGAAAATGTAGGGAAAAGTGAGTTGTTGATCTTTGTTACACCGAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

78.938

96.98

0.766
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.938

96.98

0.766
  pilQ Vibrio campbellii strain DS40M4

79.19

96.448

0.764
  pilQ Legionella pneumophila strain Lp02

42.491

96.98

0.412
  pilQ Legionella pneumophila strain ERS1305867

42.491

96.98

0.412
  pilQ Pseudomonas aeruginosa PAK

41.091

97.691

0.401
  comQ Acinetobacter baylyi ADP1

39.464

99.467

0.393
  pilQ Acinetobacter baumannii strain A118

37.197

100

0.382
  pilQ Acinetobacter baumannii D1279779

37.197

100

0.382

Multiple sequence alignment