Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCU50_RS13095 Genome accession   NZ_AP025514
Coordinates   2871981..2873006 (-) Length   341 a.a.
NCBI ID   WP_060468744.1    Uniprot ID   A0A120DG23
Organism   Vibrio toranzoniae strain CECT 7225     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2866981..2878006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU50_RS13070 aroK 2867843..2868361 (-) 519 WP_017061125.1 shikimate kinase AroK -
  OCU50_RS13075 pilQ 2868538..2870229 (-) 1692 WP_060468740.1 type IV pilus secretin PilQ Machinery gene
  OCU50_RS13080 pilP 2870289..2870807 (-) 519 WP_060468741.1 pilus assembly protein PilP Machinery gene
  OCU50_RS13085 pilO 2870794..2871405 (-) 612 WP_060468742.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU50_RS13090 pilN 2871398..2871997 (-) 600 WP_060468743.1 PilN domain-containing protein Machinery gene
  OCU50_RS13095 pilM 2871981..2873006 (-) 1026 WP_060468744.1 type IV pilus assembly protein PilM Machinery gene
  OCU50_RS13100 - 2873158..2875719 (+) 2562 WP_060468745.1 penicillin-binding protein 1A -
  OCU50_RS13105 oxyR 2875820..2876710 (-) 891 WP_060468746.1 DNA-binding transcriptional regulator OxyR -
  OCU50_RS13110 - 2876930..2877658 (+) 729 WP_017055839.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38109.69 Da        Isoelectric Point: 7.2993

>NTDB_id=92423 OCU50_RS13095 WP_060468744.1 2871981..2873006(-) (pilM) [Vibrio toranzoniae strain CECT 7225]
MGSSLITGIDINHHSIKAVVLKPVGELYAIVGYKELPISDDIFTANHTLEYQKTVKKLKELKKGLPFGSRNVAISVPDNT
VISKVLQIESDLEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRANALSKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGIDQTSLGIIPHNSTPFYKDIAFGTQELLGAGNPSDMESVFGTTQDTH
RFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQMSCESLNPMSLFENKVAQKRRSSLNCQHFG
IAAGMAMRGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=92423 OCU50_RS13095 WP_060468744.1 2871981..2873006(-) (pilM) [Vibrio toranzoniae strain CECT 7225]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGTATCAAAGCTGTGGTACTAAAACCCGTTGGGGAGTT
GTATGCCATCGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAAGAAAGGACTGCCTTTTGGAAGTCGTAACGTCGCTATTTCAGTACCAGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGATCTAGAAGATAGAGAGAAAGAGTTTTCTATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAAGAGCTGAGTTTAGATTTTGTAAAGCTCGAAGACAAACGGTTTGGTAAAGGATCGACAA
GTAGTTATCAGGTGTACGCGACTCGCAAGGAAGTTGTGGATAGTCGAGCCAATGCATTAAGCAAAGCCGGGTTTAAACCT
ATCGTCGTTGATACCCAAGCACATGGGCTTCTCAATATTTGGCAATTGGCTTCGCATTTGTACCCAGAAAAGAAGAACTG
GCTGCTGGTGGACGTTGGAATCGACCAAACCTCGTTAGGGATTATCCCGCATAATTCTACGCCTTTTTATAAAGACATCG
CCTTTGGTACTCAAGAGCTGTTAGGCGCAGGCAATCCAAGTGATATGGAAAGCGTGTTCGGCACCACTCAGGATACACAT
CGGTTTATTGTTAATCTTATTGAAAAGCTCAAGCGTCAACTGCAGCTCTATTCGTCGGTCAATGCGCTTCAACCTATCTC
AGGGATTTGGTTAATGGGTGAAGGAGCGAGCATGCCAATGGTCGTTGAGGAGTTAGAACGTCATTTTCAGATGAGTTGTG
AGTCACTGAACCCTATGTCTTTGTTTGAGAATAAGGTGGCCCAGAAACGCCGATCGTCATTGAATTGTCAGCACTTTGGT
ATTGCGGCGGGGATGGCAATGCGTGGCCTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)

Type IV pilus assembly protein PilM; (PilM_2)

(242-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A120DG23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.252

100

0.543
  pilM Vibrio campbellii strain DS40M4

46.491

100

0.466

Multiple sequence alignment