Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCV44_RS01275 Genome accession   NZ_AP025510
Coordinates   286376..287005 (+) Length   209 a.a.
NCBI ID   WP_139685158.1    Uniprot ID   -
Organism   Vibrio tasmaniensis strain LMG 20012     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 281376..292005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV44_RS01260 - 282050..284608 (-) 2559 WP_170213728.1 penicillin-binding protein 1A -
  OCV44_RS01265 pilM 284760..285785 (+) 1026 WP_139685156.1 type IV pilus assembly protein PilM Machinery gene
  OCV44_RS01270 pilN 285769..286383 (+) 615 WP_139685157.1 PilN domain-containing protein Machinery gene
  OCV44_RS01275 pilO 286376..287005 (+) 630 WP_139685158.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV44_RS01280 pilP 286989..287510 (+) 522 WP_390903455.1 pilus assembly protein PilP Machinery gene
  OCV44_RS01285 pilQ 287546..289264 (+) 1719 WP_139685159.1 type IV pilus secretin PilQ Machinery gene
  OCV44_RS01290 aroK 289441..289959 (+) 519 WP_009848907.1 shikimate kinase AroK -
  OCV44_RS01295 aroB 289984..291072 (+) 1089 WP_012604944.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24340.86 Da        Isoelectric Point: 4.5774

>NTDB_id=92347 OCV44_RS01275 WP_139685158.1 286376..287005(+) (pilO) [Vibrio tasmaniensis strain LMG 20012]
MANVQNRISWQNRMSWQDLDADEITEWPLLPQLLVILLLMVLIQGVGTWLYVLPLDDELQQMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNALTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDEIQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=92347 OCV44_RS01275 WP_139685158.1 286376..287005(+) (pilO) [Vibrio tasmaniensis strain LMG 20012]
ATGGCTAACGTTCAAAATCGGATTAGTTGGCAAAATAGGATGAGCTGGCAAGATCTCGATGCTGATGAGATCACCGAATG
GCCGCTGTTGCCCCAGCTGCTCGTGATTCTGTTGTTGATGGTATTGATTCAAGGTGTTGGGACTTGGCTGTATGTGCTGC
CTTTAGATGATGAACTGCAGCAGATGAAGCAGCAAGAGCAGACCTTGAAAGCCACCTTGAGGATAAAAGCGAATAAGGTG
GCAGCCTTGCCTAAGCTTCAGAGTCAGTTGGATGAGCTAACCAGTCGCTATGACTATCTGTTAGAGCAGCTGCCGGTTCA
AAAGGAACTTGCCAGTATGTTGGCCTCAGTGAATGAACTTGGTTTGGATAATGCGCTGACCTTCACGCGCATTGATTGGG
GGCAAAAGCAGAACAAAGAATTCCTCTATCGGTTGCCTCTCAATATTGAGCTAACCGGCGACTACCATGAGATTGGCGAT
TTCTCAGCAGCCATCGCAAAGCTGCCACGTATTATTAGCTTTGATGACGTGAATTGGCAGCGAGTTAGCCAAGAAAGCAG
CACGCTACATTTTAGGGTTCGAGCTTATACCTACCAGTTTAAATCGGAGGTGAATGATGAAATTCAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.684

92.344

0.708
  pilO Vibrio campbellii strain DS40M4

72.872

89.952

0.655

Multiple sequence alignment