Detailed information NTG0921937

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: VP456_RS14005
• Genome accession: NZ_CP142142
• Coordinates: 2781608..2782222 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain PD9
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2776608..2787222

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VP456_RS13975 (VP456_13940)	rpsT	2776911..2777177 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
VP456_RS13980 (VP456_13945)	holA	2777140..2778235 (-)	1096	Protein_2710	DNA polymerase III subunit delta	-
VP456_RS13985 (VP456_13950)	-	2778275..2778426 (-)	152	Protein_2711	hypothetical protein	-
VP456_RS13990 (VP456_13955)	-	2778467..2778601 (+)	135	WP_003152870.1	YqzM family protein	-
VP456_RS13995 (VP456_13960)	comEC	2778620..2780971 (-)	2352	WP_029973403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
VP456_RS14000 (VP456_13965)	-	2780972..2781541 (-)	570	WP_003152868.1	ComE operon protein 2	-
VP456_RS14005 (VP456_13970)	comEA	2781608..2782222 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
VP456_RS14010 (VP456_13975)	comER	2782281..2783102 (+)	822	WP_029973401.1	late competence protein ComER	-
VP456_RS14015 (VP456_13980)	-	2783171..2783908 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
VP456_RS14020 (VP456_13985)	rsfS	2783905..2784261 (-)	357	WP_007408260.1	ribosome silencing factor	-
VP456_RS14025 (VP456_13990)	yqeK	2784279..2784839 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
VP456_RS14030 (VP456_13995)	-	2784829..2785398 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
VP456_RS14035 (VP456_14000)	yhbY	2785409..2785699 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
VP456_RS14040 (VP456_14005)	aroE	2785693..2786535 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=921937 VP456_RS14005 WP_014418419.1 2781608..2782222(-) (comEA) [Bacillus velezensis strain PD9]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=921937 VP456_RS14005 WP_014418419.1 2781608..2782222(-) (comEA) [Bacillus velezensis strain PD9]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363