Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   AB3424_RS05735 Genome accession   NZ_CP162436
Coordinates   1156151..1156573 (-) Length   140 a.a.
NCBI ID   WP_258922346.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22699     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1151151..1161573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3424_RS05705 (AB3424_05705) gcvPA 1151371..1152717 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  AB3424_RS05710 (AB3424_05710) gcvT 1152737..1153828 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  AB3424_RS05715 (AB3424_05715) - 1153987..1154511 (-) 525 WP_001015117.1 shikimate kinase -
  AB3424_RS05720 (AB3424_05720) - 1154501..1154647 (-) 147 WP_001789879.1 hypothetical protein -
  AB3424_RS05725 (AB3424_05725) - 1154744..1154848 (-) 105 Protein_1132 type IV pilus minor pilin ComGF family protein -
  AB3424_RS05730 (AB3424_05730) - 1154901..1156073 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  AB3424_RS05735 (AB3424_05735) comGF 1156151..1156573 (-) 423 WP_258922346.1 competence type IV pilus minor pilin ComGF Machinery gene
  AB3424_RS05740 (AB3424_05740) comGE 1156491..1156790 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  AB3424_RS05745 (AB3424_05745) comGD 1156777..1157223 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB3424_RS05750 (AB3424_05750) comGC 1157201..1157512 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AB3424_RS05755 (AB3424_05755) comGB 1157526..1158596 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  AB3424_RS05760 (AB3424_05760) comGA 1158568..1159542 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3424_RS05765 (AB3424_05765) - 1159594..1160217 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AB3424_RS05770 (AB3424_05770) - 1160214..1160543 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AB3424_RS05775 (AB3424_05775) - 1160543..1161529 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 16413.66 Da        Isoelectric Point: 10.0943

>NTDB_id=921891 AB3424_RS05735 WP_258922346.1 1156151..1156573(-) (comGF) [Staphylococcus aureus strain TUM22699]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVDKVRIIV

Nucleotide


Download         Length: 423 bp        

>NTDB_id=921891 AB3424_RS05735 WP_258922346.1 1156151..1156573(-) (comGF) [Staphylococcus aureus strain TUM22699]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTTGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTG
ATAAAGTCCGTATAATTGTGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(66-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

95

0.95
  comGF Staphylococcus aureus N315

96.241

95

0.914