Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   VP456_RS02145 Genome accession   NZ_CP142142
Coordinates   381521..381958 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain PD9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 376521..386958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP456_RS02120 (VP456_02110) - 377541..378677 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  VP456_RS02125 (VP456_02115) - 378692..379126 (+) 435 WP_014416871.1 RDD family protein -
  VP456_RS02130 (VP456_02120) - 379243..379521 (+) 279 WP_263612330.1 YckD family protein -
  VP456_RS02135 (VP456_02125) - 379625..381061 (+) 1437 WP_014416872.1 glycoside hydrolase family 1 protein -
  VP456_RS02140 (VP456_02130) nin/comJ 381102..381500 (-) 399 WP_029973909.1 competence protein ComJ Regulator
  VP456_RS02145 (VP456_02135) nucA/comI 381521..381958 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  VP456_RS02150 (VP456_02140) hxlB 382289..382846 (-) 558 WP_021494205.1 6-phospho-3-hexuloisomerase -
  VP456_RS02155 (VP456_02145) hxlA 382843..383478 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  VP456_RS02160 (VP456_02150) - 383710..384072 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=921893 VP456_RS02145 WP_003156588.1 381521..381958(-) (nucA/comI) [Bacillus velezensis strain PD9]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=921893 VP456_RS02145 WP_003156588.1 381521..381958(-) (nucA/comI) [Bacillus velezensis strain PD9]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641