Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M3O54_RS15530 Genome accession   NZ_CP142004
Coordinates   3594758..3596464 (-) Length   568 a.a.
NCBI ID   WP_193395481.1    Uniprot ID   -
Organism   Xanthomonas nasturtii strain 8853     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3589758..3601464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3O54_RS15515 (M3O54_015505) sucC 3590039..3591208 (-) 1170 WP_064634549.1 ADP-forming succinate--CoA ligase subunit beta -
  M3O54_RS15520 (M3O54_015510) - 3591441..3593054 (+) 1614 WP_064634548.1 PAS domain-containing sensor histidine kinase -
  M3O54_RS15525 (M3O54_015515) pilR 3593234..3594628 (+) 1395 WP_064634547.1 sigma-54 dependent transcriptional regulator Regulator
  M3O54_RS15530 (M3O54_015520) pilB 3594758..3596464 (-) 1707 WP_193395481.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M3O54_RS15535 (M3O54_015525) galE 3596611..3597603 (-) 993 WP_239687036.1 UDP-glucose 4-epimerase GalE -
  M3O54_RS15540 (M3O54_015530) - 3597691..3599235 (-) 1545 WP_064634545.1 NAD(P)/FAD-dependent oxidoreductase -
  M3O54_RS15545 (M3O54_015535) - 3599237..3599698 (-) 462 WP_064634544.1 GtrA family protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61869.90 Da        Isoelectric Point: 5.5885

>NTDB_id=920847 M3O54_RS15530 WP_193395481.1 3594758..3596464(-) (pilB) [Xanthomonas nasturtii strain 8853]
MGITGLARRLVQDGALDESAARGAMDQAAAAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQNAVKLVSD
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQANNASFGSSMGDDDEEMGDL
DVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLSQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSALPEHALLAEGF
TPEQIANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGTIVLEGGNAMQIAEAAQKIGIRDLRQSALMKAAHGVTSLA
EINRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=920847 M3O54_RS15530 WP_193395481.1 3594758..3596464(-) (pilB) [Xanthomonas nasturtii strain 8853]
ATCGGTATTACCGGCTTGGCCCGCCGCTTGGTTCAAGACGGTGCGCTCGATGAATCTGCCGCACGCGGGGCCATGGACCA
AGCTGCAGCCGCCAAGGTTCCGCTTCCTCAGTGGTTTGCCGAAAAGAAACTTGTATCGGCAGCGCAGCTGGCTGCCGCCA
ATGCCGTGGAGTTTGGCATGCCGCTGATGGATGTGTCTGTGTTCGACGCTAGCCAGAACGCGGTGAAGTTAGTCAGTGAT
GAGTTGCTCCAGAAACATCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCCTGGTGGACGAGGATCAGATCCGCC
GCACCTTGGAGCAATGGCAGGCCAATAACGCTTCGTTCGGCTCGTCAATGGGGGACGATGACGAAGAAATGGGCGATCTG
GACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGGGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGGTTGCGCATCGATGGGCTACTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAGCCAGCGCATCGCGGCG
CGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGATGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTGAGTACCTTGCCGACCCTGTTCGGTGAGAAGGTGGTGTTGCGTATTCTGGACGCCA
GTGCGGCCAAGCTGGGGATCGAGAAGCTGGGCTATGAGGCGGATCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCC
TACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTTGTATACGGCGCTGGGTATCCTCAACGA
TGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTTGAAATCCGCTTGCCGGGCGTCAATCAGGTGCAGCAGAACGTCA
AGCGCGGCATGACGTTTGCGGCTGCGTTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGTTGTCGACGCTGCATACCAACGATGC
GCCGCAGACCATCGCGCGTTTGATGAATATGGGGATTGCGCCGTACAACATCACCTCGTCGGTGACGCTGGTCATCGCGC
AGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGGCCCTGCCGGAGCATGCGCTGTTGGCGGAAGGCTTT
ACGCCCGAGCAAATCGCAAACATCGAGCTGTATGAGGCGGTCGGCTGCGATGAATGCACCGAAGGTTACAAGGGACGTAC
CGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTACGATCGTGCTGGAAGGCGGTAACGCGATGCAGATCGCCG
AGGCTGCGCAAAAGATCGGCATCCGGGACTTGCGCCAGTCCGCGTTGATGAAGGCGGCGCATGGCGTGACCAGCCTGGCT
GAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-142)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

100

0.562
  pilB Acinetobacter baylyi ADP1

55.634

100

0.556
  pilB Legionella pneumophila strain ERS1305867

52.289

100

0.523
  pilF Neisseria gonorrhoeae MS11

48.944

100

0.489
  pilB Vibrio cholerae strain A1552

48.596

100

0.488
  pilB Vibrio campbellii strain DS40M4

46.397

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.454

99.296

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.048

92.43

0.361