Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCU78_RS13160 Genome accession   NZ_AP025490
Coordinates   2998809..2999402 (-) Length   197 a.a.
NCBI ID   WP_137375092.1    Uniprot ID   -
Organism   Vibrio gallaecicus strain CECT 7244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2993809..3004402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU78_RS13140 aroB 2994727..2995815 (-) 1089 WP_137375096.1 3-dehydroquinate synthase -
  OCU78_RS13145 aroK 2995840..2996358 (-) 519 WP_137375095.1 shikimate kinase AroK -
  OCU78_RS13150 pilQ 2996535..2998247 (-) 1713 WP_137375094.1 type IV pilus secretin PilQ Machinery gene
  OCU78_RS13155 pilP 2998307..2998822 (-) 516 WP_137375093.1 pilus assembly protein PilP Machinery gene
  OCU78_RS13160 pilO 2998809..2999402 (-) 594 WP_137375092.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU78_RS13165 - 2999533..3000099 (-) 567 Protein_2514 PilN domain-containing protein -
  OCU78_RS13170 pilM 3000083..3001105 (-) 1023 WP_137375090.1 type IV pilus assembly protein PilM Machinery gene
  OCU78_RS13175 - 3001310..3003955 (+) 2646 WP_167494071.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23067.52 Da        Isoelectric Point: 4.6739

>NTDB_id=92067 OCU78_RS13160 WP_137375092.1 2998809..2999402(-) (pilO) [Vibrio gallaecicus strain CECT 7244]
MVSLQELDIDEITEWPLFPQVLVVLLLMFIIQGFGVWFYLMPQNDELEGLKQQEQTLKTTLRIKANKVATLPQLQKQLNE
LTSRYDYLLQQLPVQKELASMLAAVNELGLDNKLTFTRIDWGEKQNKEFLYRLPLNIELTGDYHEIGDFSAAIAKLPRII
SFDDVNWQRVSQESSTLHFRVRAYTYQFKPEVRDEAQ

Nucleotide


Download         Length: 594 bp        

>NTDB_id=92067 OCU78_RS13160 WP_137375092.1 2998809..2999402(-) (pilO) [Vibrio gallaecicus strain CECT 7244]
ATGGTTAGTTTACAAGAACTTGATATTGATGAAATTACAGAGTGGCCGTTATTTCCTCAAGTGCTCGTGGTTTTATTACT
GATGTTCATTATCCAAGGTTTTGGCGTTTGGTTTTATTTGATGCCACAAAATGATGAGTTAGAGGGGCTAAAGCAACAAG
AGCAAACATTGAAAACGACGTTGCGAATTAAAGCGAATAAAGTTGCCACCTTGCCTCAACTGCAAAAGCAGCTAAATGAG
TTAACTAGCCGTTATGACTACTTACTGCAGCAATTGCCTGTTCAAAAAGAGTTGGCAAGCATGTTGGCTGCGGTTAATGA
ACTGGGGCTGGATAATAAGCTGACTTTTACTCGCATTGATTGGGGAGAAAAACAAAATAAAGAGTTTCTTTATCGCTTAC
CCCTCAATATCGAACTAACGGGTGATTACCATGAAATTGGTGATTTTTCCGCAGCAATTGCCAAGCTACCAAGAATTATC
AGTTTTGACGATGTGAATTGGCAACGAGTGAGCCAAGAAAGTAGCACGCTTCACTTTCGTGTCAGAGCTTATACCTACCA
GTTTAAACCGGAGGTCAGAGATGAAGCCCAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.923

98.985

0.761
  pilO Vibrio campbellii strain DS40M4

72.105

96.447

0.695

Multiple sequence alignment