Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCU78_RS13170 Genome accession   NZ_AP025490
Coordinates   3000083..3001105 (-) Length   340 a.a.
NCBI ID   WP_137375090.1    Uniprot ID   -
Organism   Vibrio gallaecicus strain CECT 7244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2995083..3006105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU78_RS13145 aroK 2995840..2996358 (-) 519 WP_137375095.1 shikimate kinase AroK -
  OCU78_RS13150 pilQ 2996535..2998247 (-) 1713 WP_137375094.1 type IV pilus secretin PilQ Machinery gene
  OCU78_RS13155 pilP 2998307..2998822 (-) 516 WP_137375093.1 pilus assembly protein PilP Machinery gene
  OCU78_RS13160 pilO 2998809..2999402 (-) 594 WP_137375092.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCU78_RS13165 - 2999533..3000099 (-) 567 Protein_2514 PilN domain-containing protein -
  OCU78_RS13170 pilM 3000083..3001105 (-) 1023 WP_137375090.1 type IV pilus assembly protein PilM Machinery gene
  OCU78_RS13175 - 3001310..3003955 (+) 2646 WP_167494071.1 penicillin-binding protein 1A -
  OCU78_RS13180 oxyR 3004112..3005029 (-) 918 WP_137375089.1 DNA-binding transcriptional regulator OxyR -
  OCU78_RS13185 - 3005251..3005979 (+) 729 WP_137375088.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37885.35 Da        Isoelectric Point: 7.0346

>NTDB_id=92068 OCU78_RS13170 WP_137375090.1 3000083..3001105(-) (pilM) [Vibrio gallaecicus strain CECT 7244]
MGSSSVIGIDISSHSIKAVVLKPVGELYALIGYKELPISDDIFSDNHTFEYQKIVKKLKELKKELPLFSRKVAISIPDNS
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEDLSLDFVKVEEKRFGKSSTTSYQVYATRKEVVESRSLAIKKAGLEP
VVVAIHAHGLLNIWQLASQLNPDKKNWLLVDVGLNQLSLGIMPQGGSSFYKDISFGSHSTNSTGEGHPASAFGSAEENQK
YINDFIEKLKRQLQLYSSVNSTHPLSGIWLMGESANTPMLIEELERQLGLSCELLNPLSLFENKLSKKRRMHTDWQSFGI
AAGMAISGLKWGEVKHVASS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=92068 OCU78_RS13170 WP_137375090.1 3000083..3001105(-) (pilM) [Vibrio gallaecicus strain CECT 7244]
ATGGGTTCATCATCAGTCATCGGTATAGATATAAGTAGCCACAGTATTAAAGCCGTGGTTCTCAAACCCGTAGGGGAGTT
ATATGCATTAATTGGATATAAAGAGCTTCCTATTTCGGACGACATTTTCTCTGATAATCATACCTTCGAGTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTTAAAAAAGAGTTACCTTTGTTTAGTCGAAAGGTCGCCATTTCCATCCCTGACAATTCT
GTTATTAGCAAAGTACTTCAAATTGAAAGCGAACTGGAAGATAGAGAAAAAGAGTTCTCTATTTACCAAACCTTCGCTCA
TCAATCTCCATTTCCAATCGAAGACCTCAGTTTAGATTTCGTCAAAGTTGAAGAAAAACGCTTTGGTAAAAGTTCAACGA
CAAGCTATCAGGTCTACGCGACTCGAAAAGAAGTCGTAGAAAGTCGGTCTTTGGCTATAAAGAAAGCAGGGCTAGAACCT
GTTGTTGTGGCTATTCATGCTCATGGGTTATTGAATATCTGGCAATTAGCGTCTCAGTTAAATCCAGATAAAAAAAATTG
GCTACTTGTTGATGTAGGGCTGAATCAACTGTCTTTGGGGATTATGCCCCAGGGTGGTTCTTCTTTTTATAAAGACATTT
CATTTGGTAGCCACTCAACAAACTCAACGGGCGAAGGTCACCCTGCGAGTGCATTTGGTAGTGCCGAAGAAAATCAAAAA
TACATAAATGATTTCATTGAGAAGCTAAAGCGGCAACTACAACTTTATTCTTCAGTTAACTCTACGCACCCTCTTTCTGG
AATTTGGTTGATGGGGGAAAGTGCAAATACACCAATGCTAATTGAAGAGCTCGAAAGGCAGTTAGGTTTGAGTTGTGAGC
TTCTGAACCCTCTTTCTCTATTTGAGAATAAATTATCTAAAAAACGTCGCATGCATACTGACTGGCAAAGTTTTGGCATC
GCTGCTGGAATGGCTATCAGTGGGCTTAAATGGGGAGAGGTGAAGCATGTTGCATCAAGTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.353

100

0.538
  pilM Vibrio campbellii strain DS40M4

49.693

95.882

0.476

Multiple sequence alignment