Detailed information NTG0920550

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VK748_RS12630
• Genome accession: NZ_CP141948
• Coordinates: 2700044..2701108 (+)
• Length: 354 a.a.
• NCBI ID: WP_009613650.1
• Uniprot ID: A0A1A5D9E0
• Organism: Pseudomonas citronellolis strain G5.41a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2695044..2706108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VK748_RS12615 (VK748_12615)	-	2695067..2695837 (+)	771	WP_324711067.1	class I SAM-dependent methyltransferase	-
VK748_RS12620 (VK748_12620)	-	2695899..2696390 (+)	492	WP_324711068.1	M48 family metallopeptidase	-
VK748_RS12625 (VK748_12625)	-	2697426..2699858 (-)	2433	WP_043268466.1	penicillin-binding protein 1A	-
VK748_RS12630 (VK748_12630)	pilM	2700044..2701108 (+)	1065	WP_009613650.1	pilus assembly protein PilM	Machinery gene
VK748_RS12635 (VK748_12635)	pilN	2701108..2701707 (+)	600	WP_009613648.1	type 4a pilus biogenesis protein PilN	-
VK748_RS12640 (VK748_12640)	pilO	2701704..2702360 (+)	657	WP_009613645.1	type 4a pilus biogenesis protein PilO	-
VK748_RS12645 (VK748_12645)	pilP	2702357..2702881 (+)	525	WP_009613643.1	type 4a pilus biogenesis lipoprotein PilP	-
VK748_RS12650 (VK748_12650)	pilQ	2702938..2705025 (+)	2088	WP_324711070.1	type IV pilus secretin PilQ	Machinery gene
VK748_RS12655 (VK748_12655)	aroK	2705034..2705552 (+)	519	WP_324711071.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37773.09 Da
• Isoelectric Point: 4.4526

>NTDB_id=920550 VK748_RS12630 WP_009613650.1 2700044..2701108(+) (pilM) [Pseudomonas citronellolis strain G5.41a]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNSVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKTIEMDGGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=920550 VK748_RS12630 WP_009613650.1 2700044..2701108(+) (pilM) [Pseudomonas citronellolis strain G5.41a]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAACTCCTCGAGTT
GAGCCGTTCGGGCGGGCGCTACAAGGTAGAGGCCTACGCCGTAGAGCCGCTCCCGCCGAACTCGGTGGTCGAGAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGTGTGGTCGCCAAGGCGCGCACCGGCGTGAAGACGGCTGCCGTG
GCGGTCGCCGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGGCGGTCTTTCCGAAGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGCGTATCCGCGC
GCAACGCCGAGCGCGTCGACGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTCACCGCCAAGGTGGTGGACGTCGAGGCCTACGCGCTGGAGCGCGCCTTCGGCCTGCTCGGCGACCAACTGGG
CGGCAACGCCGACGAGCTGACCGTCGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTCCACCACGGCC
GCACCATCTATACCCGCGAGCAGCTGTTCGGCGGGCGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCGGTG
GAAGAAGCCGGCCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAACCGTTCAAGGATGC
GGTGGTGCAGCAGGTTTCGCGCTCCCTGCAGTTCTTCTTCGCCGCGGGCCAGTTCAACGACGTCGACTACATCCTGCTCG
CCGGCGGCACGGCTTCCATTCCCGACCTGGACCGGCTCATCCAGCAGAAGATCGGCACCCCGACGCTGGTGGCCAACCCC
TTCGCCGACATGGTGCTGAGCAACAAGGTGAATGCCGGGGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1A5D9E0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46