Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCV50_RS13225 Genome accession   NZ_AP025487
Coordinates   2971037..2971630 (-) Length   197 a.a.
NCBI ID   WP_239841916.1    Uniprot ID   -
Organism   Vibrio fortis strain LMG 21557     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2966037..2976630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV50_RS23305 - 2966499..2966885 (-) 387 Protein_2509 ATP-binding protein -
  OCV50_RS13205 aroB 2966968..2968056 (-) 1089 WP_261903240.1 3-dehydroquinate synthase -
  OCV50_RS13210 aroK 2968081..2968599 (-) 519 WP_032550005.1 shikimate kinase AroK -
  OCV50_RS13215 - 2968774..2970402 (-) 1629 Protein_2512 type IV pilus secretin PilQ -
  OCV50_RS13220 pilP 2970532..2971050 (-) 519 WP_261903242.1 pilus assembly protein PilP Machinery gene
  OCV50_RS13225 pilO 2971037..2971630 (-) 594 WP_239841916.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV50_RS13230 pilN 2971623..2972222 (-) 600 WP_261903243.1 PilN domain-containing protein Machinery gene
  OCV50_RS13235 pilM 2972206..2973231 (-) 1026 WP_261903244.1 type IV pilus assembly protein PilM Machinery gene
  OCV50_RS13240 - 2973379..2975937 (+) 2559 WP_261903245.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22902.29 Da        Isoelectric Point: 4.4977

>NTDB_id=92021 OCV50_RS13225 WP_239841916.1 2971037..2971630(-) (pilO) [Vibrio fortis strain LMG 21557]
MVNFQDLDVDEITEWPLLPQLLVIVLLMLIIQGFGAWFYVMPLNDELEQMKQQEQTLKTTLRIKANKVATLPALQNQLDE
LTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGEKQNKAFLYSLPLNIELTGDYHEIGDFSAAIAKLPRII
NFDDVTWQRVSQESSTLHFRVRAYTYQFKAEVKDESR

Nucleotide


Download         Length: 594 bp        

>NTDB_id=92021 OCV50_RS13225 WP_239841916.1 2971037..2971630(-) (pilO) [Vibrio fortis strain LMG 21557]
ATGGTTAATTTTCAAGATCTCGATGTCGATGAAATTACGGAGTGGCCGCTGTTACCGCAGCTGTTGGTGATCGTTTTACT
GATGTTAATCATCCAAGGCTTCGGAGCTTGGTTCTATGTAATGCCACTCAATGACGAGTTAGAGCAGATGAAACAGCAAG
AGCAGACGCTAAAAACCACCCTTCGAATTAAAGCCAATAAAGTGGCCACTTTGCCTGCGTTGCAGAATCAACTCGATGAA
TTGACCAGTCGTTACGACTATCTACTTGAACAGTTACCAGTGCAAAAAGAGTTGGCGAGTATGCTTGCCTCGGTTAACGA
GCTCGGTCTAGATAACAAACTCACCTTTACACGCATTGATTGGGGCGAAAAACAGAACAAGGCCTTCTTATATAGCTTAC
CTCTGAATATCGAGCTGACAGGTGATTACCATGAAATTGGTGACTTTTCCGCAGCAATAGCCAAGTTGCCGCGCATCATT
AACTTTGATGATGTGACTTGGCAGAGGGTCAGCCAAGAGAGCAGTACTCTGCATTTTCGTGTTCGCGCATACACCTACCA
ATTTAAGGCAGAGGTGAAAGATGAATCTCGTTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.897

98.985

0.751
  pilO Vibrio campbellii strain DS40M4

72.487

95.939

0.695

Multiple sequence alignment