Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV50_RS13235 Genome accession   NZ_AP025487
Coordinates   2972206..2973231 (-) Length   341 a.a.
NCBI ID   WP_261903244.1    Uniprot ID   -
Organism   Vibrio fortis strain LMG 21557     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2967206..2978231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV50_RS13210 aroK 2968081..2968599 (-) 519 WP_032550005.1 shikimate kinase AroK -
  OCV50_RS13215 - 2968774..2970402 (-) 1629 Protein_2512 type IV pilus secretin PilQ -
  OCV50_RS13220 pilP 2970532..2971050 (-) 519 WP_261903242.1 pilus assembly protein PilP Machinery gene
  OCV50_RS13225 pilO 2971037..2971630 (-) 594 WP_239841916.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV50_RS13230 pilN 2971623..2972222 (-) 600 WP_261903243.1 PilN domain-containing protein Machinery gene
  OCV50_RS13235 pilM 2972206..2973231 (-) 1026 WP_261903244.1 type IV pilus assembly protein PilM Machinery gene
  OCV50_RS13240 - 2973379..2975937 (+) 2559 WP_261903245.1 penicillin-binding protein 1A -
  OCV50_RS13245 oxyR 2976025..2976912 (-) 888 WP_239841920.1 DNA-binding transcriptional regulator OxyR -
  OCV50_RS13250 - 2977118..2977846 (+) 729 WP_239841921.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38246.85 Da        Isoelectric Point: 7.9448

>NTDB_id=92023 OCV50_RS13235 WP_261903244.1 2972206..2973231(-) (pilM) [Vibrio fortis strain LMG 21557]
MGSSLVTGIDINNHSIKAVVLKPVGELYALVGYKELPITDDIFTDNHTLEYQKTVKKLKELRKGLPFGCRHAAISVPDNT
VISKILQIESDLEDREKEFSIYQTFAHQSPFPIEELSLDFIKLDDKRFGKGSTTSYQVYATRKEVVESRSQALTKAGFRP
IVVDTQAHGLLNIWQLATRLYPEKQSWIIIDIGVNQTSLGLIPQSGVPFFKDIAFGTQALYQSEYFGDVSSPFGSAEQTQ
AFTVNLIEKLKRQFQLYASVNSLQALRGIWLMGEGAETPMLVEQLERHFQLACEPLNPLALFENKASTKRYKNMDRHVFG
IAAGMAVSGLKWRGVKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=92023 OCV50_RS13235 WP_261903244.1 2972206..2973231(-) (pilM) [Vibrio fortis strain LMG 21557]
ATGGGTTCGTCATTAGTTACAGGTATAGATATAAACAATCACAGCATCAAAGCTGTGGTTTTAAAGCCCGTTGGAGAGTT
ATATGCCTTAGTGGGGTACAAAGAACTGCCTATTACGGACGACATTTTCACAGATAACCACACCTTGGAGTATCAGAAAA
CTGTAAAGAAACTCAAAGAACTTAGGAAAGGCTTACCGTTTGGCTGTCGTCATGCGGCCATCTCAGTCCCAGATAATACC
GTGATTAGCAAAATCTTGCAGATAGAAAGTGACCTGGAAGACCGAGAAAAAGAGTTCTCTATCTATCAAACCTTCGCTCA
TCAATCGCCTTTCCCGATTGAGGAACTGAGCTTGGATTTCATTAAATTGGATGATAAGCGATTTGGTAAAGGTTCAACCA
CCAGTTATCAGGTTTATGCGACGCGCAAAGAGGTGGTGGAAAGCCGCTCTCAAGCGCTGACTAAAGCAGGCTTTCGTCCA
ATTGTGGTCGACACTCAAGCCCATGGACTGCTCAACATTTGGCAGTTGGCAACTCGACTTTATCCAGAGAAACAGAGTTG
GATCATCATCGATATTGGCGTGAATCAAACCTCGCTCGGGCTTATCCCTCAAAGTGGGGTGCCTTTCTTTAAAGACATCG
CATTTGGTACTCAAGCGCTCTATCAGTCAGAGTATTTCGGAGATGTTTCAAGCCCTTTTGGTTCAGCAGAGCAGACCCAA
GCCTTTACCGTGAATCTGATCGAAAAACTCAAACGGCAGTTTCAGCTCTATGCGTCGGTTAACTCGTTGCAGGCGTTACG
AGGGATTTGGTTGATGGGAGAAGGGGCAGAAACACCGATGCTCGTCGAGCAACTAGAGCGTCATTTTCAGTTGGCTTGTG
AGCCTCTTAACCCATTAGCCCTGTTTGAAAATAAGGCTTCTACTAAGCGTTATAAGAATATGGATCGTCATGTCTTTGGT
ATTGCTGCAGGGATGGCCGTGAGTGGCTTGAAATGGCGAGGAGTGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

52.059

99.707

0.519
  pilM Vibrio campbellii strain DS40M4

47.384

100

0.478

Multiple sequence alignment