Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   VLW43_RS22710 Genome accession   NZ_CP141736
Coordinates   4636576..4638096 (-) Length   506 a.a.
NCBI ID   WP_000058962.1    Uniprot ID   A0A3A6THD0
Organism   Escherichia coli strain 2023CK-01509     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4631576..4643096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VLW43_RS22700 (VLW43_22700) hdfR 4635255..4636094 (-) 840 WP_000379245.1 HTH-type transcriptional regulator HdfR -
  VLW43_RS22705 (VLW43_22705) maoP 4636213..4636551 (+) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  VLW43_RS22710 (VLW43_22710) comM 4636576..4638096 (-) 1521 WP_000058962.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  VLW43_RS22715 (VLW43_22715) ilvL 4638449..4638547 (+) 99 WP_001311244.1 ilv operon leader peptide -
  VLW43_RS22720 (VLW43_22720) ilvX 4638634..4638684 (+) 51 WP_001387183.1 peptide IlvX -
  VLW43_RS22725 (VLW43_22725) ilvG 4638687..4640333 (+) 1647 WP_001012609.1 acetolactate synthase 2 catalytic subunit -
  VLW43_RS22730 (VLW43_22730) ilvM 4640330..4640593 (+) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  VLW43_RS22735 (VLW43_22735) ilvE 4640613..4641542 (+) 930 WP_000208520.1 branched-chain-amino-acid transaminase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55118.30 Da        Isoelectric Point: 7.3874

>NTDB_id=919109 VLW43_RS22710 WP_000058962.1 4636576..4638096(-) (comM) [Escherichia coli strain 2023CK-01509]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWQQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=919109 VLW43_RS22710 WP_000058962.1 4636576..4638096(-) (comM) [Escherichia coli strain 2023CK-01509]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACTGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTGGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTATCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGTGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCAGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCATCGTTAACTGCGATGGTAGGCGGTGGCGCAATTCCAGGGCCCGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6THD0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.904

100

0.595
  comM Vibrio cholerae strain A1552

59.562

99.209

0.591
  comM Glaesserella parasuis strain SC1401

58.777

100

0.589
  comM Vibrio campbellii strain DS40M4

58.367

99.209

0.579
  comM Legionella pneumophila str. Paris

47.791

98.419

0.47
  comM Legionella pneumophila strain ERS1305867

47.791

98.419

0.47
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443