Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN28_RS02825 Genome accession   NZ_CP141725
Coordinates   573999..575018 (-) Length   339 a.a.
NCBI ID   WP_338998085.1    Uniprot ID   -
Organism   Lactococcus formosensis strain M03102409 EE5d7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 568999..580018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN28_RS02795 (VNN28_02795) - 570561..571430 (+) 870 WP_338997824.1 RluA family pseudouridine synthase -
  VNN28_RS02800 (VNN28_02800) - 571517..572362 (-) 846 WP_017368750.1 zinc ABC transporter substrate-binding protein -
  VNN28_RS02805 (VNN28_02805) comGF 572598..573038 (-) 441 WP_017368749.1 competence type IV pilus minor pilin ComGF -
  VNN28_RS02810 (VNN28_02810) comGE 573019..573252 (-) 234 WP_243416060.1 competence type IV pilus minor pilin ComGE -
  VNN28_RS02815 (VNN28_02815) comGD 573284..573700 (-) 417 WP_165711775.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN28_RS02820 (VNN28_02820) comYC 573675..573980 (-) 306 WP_017368746.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN28_RS02825 (VNN28_02825) comGB 573999..575018 (-) 1020 WP_338998085.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN28_RS02830 (VNN28_02830) comGA 574957..575901 (-) 945 WP_338997825.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN28_RS02835 (VNN28_02835) rpsU 576088..576264 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN28_RS02840 (VNN28_02840) guaB 576389..577870 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN28_RS02845 (VNN28_02845) - 578044..579648 (+) 1605 WP_338997826.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39392.03 Da        Isoelectric Point: 9.2554

>NTDB_id=918989 VNN28_RS02825 WP_338998085.1 573999..575018(-) (comGB) [Lactococcus formosensis strain M03102409 EE5d7]
MDLSQLLQLRVKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFYEDI
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRRKLIQVSTYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYAIQNLPTI
FLGCLFGLLVFFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGVEFLQILDSGQKFEDALRFYPIFTKELALIVEYGELKAKLGQELMVFSEESWSLFFERVERAMQLIQPIVFLLVGLLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918989 VNN28_RS02825 WP_338998085.1 573999..575018(-) (comGB) [Lactococcus formosensis strain M03102409 EE5d7]
ATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAAACTTATTCAACTCATGAA
TAATCTTTTCAAAAGTGGTTTCCACCTCTCTGAAATTATTGATTTTCTTGAACGGTCGGGATTAACGGAGGCATATTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAATGGACAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTTATGAGGATATT
GTGACTCAGCTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTGGGGCTGATTGAAGAAAAACTGATACGCGT
TTTGAACATCCGCAGAAAGCTCATCCAAGTCTCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GCTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGCTATACAAAATCTTCCGACCATC
TTTTTAGGGTGCCTCTTCGGTTTGCTTGTTTTCTTTTACCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTTAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGACTTACGTCAGATTTGTTTCATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTGTTGAATTCCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCTCTTCGATTTTACCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGGCTAAGCTAGGGCAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTCGAAAGAGCAATGCAACTTATCCAGCCTATTGTTTTCTTGCTGGTGGGTTTGTTGATT
ATATTAATTTATGCTGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.959

99.705

0.528
  comGB/cglB Streptococcus mitis NCTC 12261

52.239

98.82

0.516
  comGB/cglB Streptococcus mitis SK321

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae D39

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae R6

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae TIGR4

51.642

98.82

0.51
  comGB/cglB Streptococcus pneumoniae Rx1

51.642

98.82

0.51
  comYB Streptococcus gordonii str. Challis substr. CH1

51.198

98.525

0.504
  comYB Streptococcus mutans UA140

50.476

92.92

0.469
  comYB Streptococcus mutans UA159

50.159

92.92

0.466