Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN27_RS02885 Genome accession   NZ_CP141700
Coordinates   591712..592731 (-) Length   339 a.a.
NCBI ID   WP_268864970.1    Uniprot ID   -
Organism   Lactococcus petauri strain R22-25 IC35     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 586712..597731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN27_RS02855 (VNN27_02855) - 588279..589163 (+) 885 WP_086582775.1 RluA family pseudouridine synthase -
  VNN27_RS02860 (VNN27_02860) - 589234..590079 (-) 846 WP_019336001.1 zinc ABC transporter substrate-binding protein -
  VNN27_RS02865 (VNN27_02865) comGF 590311..590751 (-) 441 WP_019336002.1 competence type IV pilus minor pilin ComGF -
  VNN27_RS02870 (VNN27_02870) comGE 590732..590965 (-) 234 WP_346350127.1 competence type IV pilus minor pilin ComGE -
  VNN27_RS02875 (VNN27_02875) comGD 590997..591413 (-) 417 WP_019293010.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN27_RS02880 (VNN27_02880) comYC 591388..591693 (-) 306 WP_040087209.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN27_RS02885 (VNN27_02885) comGB 591712..592731 (-) 1020 WP_268864970.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN27_RS02890 (VNN27_02890) comGA 592670..593614 (-) 945 WP_019293012.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN27_RS02895 (VNN27_02895) rpsU 593800..593976 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN27_RS02900 (VNN27_02900) guaB 594102..595583 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN27_RS02905 (VNN27_02905) - 595752..597353 (+) 1602 WP_346350128.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39396.98 Da        Isoelectric Point: 9.4975

>NTDB_id=918494 VNN27_RS02885 WP_268864970.1 591712..592731(-) (comGB) [Lactococcus petauri strain R22-25 IC35]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRKKLVQVATYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTL
FLGWLFSLFVLFYLSRYYWKKKTALEALRLLVKIPFVGRFVQLYLTAYFAREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDSGQKFEDALRFYPIFTKELSLIVEYGELKSKLGKELMIFSEESWSLFFERIERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918494 VNN27_RS02885 WP_268864970.1 591712..592731(-) (comGB) [Lactococcus petauri strain R22-25 IC35]
ATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAAGCTCATTCAACTCATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTCTCTGAAATCATTGATTTCCTTGAACGATCGGGTCTTACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTTAATGGGCAAAGTTTCTCTGGTATTTTGGCGAAGCTAAATTTTTATGAGGATGTT
GTGACTCAGCTCTCTTTGGCTGAGTCACATGGGAATGTTGAGTATACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAAAAAGCTTGTCCAAGTTGCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATTATGCTTG
GATTGAAAAATTATTTACTACCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCCTC
TTTTTAGGGTGGCTCTTCAGTTTGTTCGTTTTATTTTATCTGTCCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GCTGAGGCTACTGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTGCAAGAGAATGGG
GAAACTTAATTGCTCAAGCTGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATTTTCAAAGAA
TTCGGTTTTGAGCTTCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCTCTTCGATTTTACCCCATATTTACCAA
GGAGCTCTCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTGATGATTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGGATTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCGGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.869

99.115

0.534
  comYB Streptococcus gordonii str. Challis substr. CH1

53.293

98.525

0.525
  comGB/cglB Streptococcus pneumoniae Rx1

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae D39

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae R6

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae TIGR4

52.836

98.82

0.522
  comGB/cglB Streptococcus mitis SK321

52.836

98.82

0.522
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comYB Streptococcus mutans UA140

52.866

92.625

0.49
  comYB Streptococcus mutans UA159

52.548

92.625

0.487