Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV39_RS01170 Genome accession   NZ_AP025472
Coordinates   262886..263977 (+) Length   363 a.a.
NCBI ID   WP_261888788.1    Uniprot ID   -
Organism   Vibrio cortegadensis strain CECT 7227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 257886..268977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV39_RS01155 - 258266..258997 (-) 732 WP_261888786.1 glutathione peroxidase -
  OCV39_RS01160 oxyR 259165..260058 (+) 894 WP_017052581.1 DNA-binding transcriptional regulator OxyR -
  OCV39_RS01165 - 260166..262742 (-) 2577 WP_261888787.1 penicillin-binding protein 1A -
  OCV39_RS01170 pilM 262886..263977 (+) 1092 WP_261888788.1 type IV pilus assembly protein PilM Machinery gene
  OCV39_RS01175 pilN 263964..264599 (+) 636 WP_261888789.1 PilN domain-containing protein Machinery gene
  OCV39_RS01180 pilO 264592..265182 (+) 591 WP_017052577.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV39_RS01185 pilP 265172..265696 (+) 525 WP_390903227.1 pilus assembly protein PilP Machinery gene
  OCV39_RS01190 pilQ 265796..267592 (+) 1797 WP_261888790.1 type IV pilus secretin PilQ Machinery gene
  OCV39_RS01195 aroK 267777..268295 (+) 519 WP_017052574.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40487.24 Da        Isoelectric Point: 7.6659

>NTDB_id=91814 OCV39_RS01170 WP_261888788.1 262886..263977(+) (pilM) [Vibrio cortegadensis strain CECT 7227]
MGKSLITGIDIGHHSIKAVVLKSMGGRYALMSYQELPITDGIVADNHTLNYQKIVKKLNELRKGLPLFSRQVAISIPDNA
VISKVLQIDSELQDRELEFALYQAFSQQSPFPIEEVSLDFIKLDDKHRLKVGTLPASIDSASRTPRTSPHASTSHASMTS
YQVYATRKEVVASRAEVVLKAGMTPYVVDVQAHSLLQVWQLLAHQHPDKKNWMLVDVGLTQISLCSIPEGKSPFHKDIAY
GTRHAKSLDDNPSLDAASDHNVDRFFQEVFERIRRQLQLYASVNNQKTVEGIWLSGGGANIPMLSEHLSRELSLDCEILN
LFSLFEDKTSKRKVLRADSQVYSTAAGLAVSGIQWMEAAHVRQ

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=91814 OCV39_RS01170 WP_261888788.1 262886..263977(+) (pilM) [Vibrio cortegadensis strain CECT 7227]
ATGGGTAAATCATTAATTACTGGTATTGATATCGGGCATCACAGCATTAAAGCGGTAGTACTGAAATCAATGGGTGGACG
TTATGCTCTGATGAGTTATCAAGAGTTGCCTATTACAGATGGTATTGTCGCTGATAACCACACCCTGAATTATCAGAAAA
TTGTAAAGAAATTAAACGAACTAAGAAAGGGGCTACCTCTATTTAGTCGTCAAGTCGCTATTTCGATCCCGGACAATGCC
GTAATCAGCAAGGTGTTGCAAATCGACAGTGAGCTACAAGATAGAGAGCTCGAATTCGCGCTTTATCAAGCCTTTTCCCA
GCAGTCTCCATTTCCGATTGAAGAGGTCAGTTTAGATTTTATCAAACTTGATGATAAACATAGGCTTAAAGTGGGCACTC
TGCCAGCGTCAATCGATTCGGCCTCTCGAACACCACGAACTTCCCCTCATGCTTCGACCTCTCACGCTTCGATGACGAGC
TATCAAGTGTACGCGACTCGTAAAGAGGTGGTAGCAAGCCGTGCTGAAGTAGTACTGAAGGCAGGAATGACACCTTATGT
GGTGGACGTTCAGGCTCATAGCTTATTGCAAGTATGGCAGTTGCTCGCTCATCAACATCCAGATAAGAAAAATTGGATGC
TGGTGGATGTCGGTTTAACACAGATCTCGCTGTGCAGCATTCCTGAGGGAAAATCGCCTTTCCATAAAGATATCGCTTAT
GGGACTCGCCACGCCAAATCTTTAGATGACAACCCATCACTTGATGCCGCTTCCGATCATAACGTAGACCGATTTTTCCA
AGAAGTATTTGAGCGGATTCGGCGACAGCTTCAACTTTATGCATCGGTGAATAATCAGAAAACGGTGGAGGGTATCTGGC
TGTCTGGAGGAGGGGCAAACATTCCCATGCTGTCGGAGCATTTGAGTCGAGAATTGAGCCTAGATTGTGAAATTTTAAAC
CTTTTTAGTCTGTTTGAGGATAAGACATCGAAACGGAAGGTGCTTAGGGCAGATAGCCAGGTATACAGCACAGCTGCGGG
GTTGGCGGTGAGTGGGATTCAATGGATGGAGGCGGCTCATGTTAGACAGTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(157-243)

Type IV pilus assembly protein PilM; (PilM_2)

(8-126)

Type IV pilus assembly protein PilM; (PilM_2)

(265-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.93

100

0.548
  pilM Vibrio campbellii strain DS40M4

46.779

98.347

0.46

Multiple sequence alignment