Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCV20_RS01230 Genome accession   NZ_AP025470
Coordinates   274448..275059 (+) Length   203 a.a.
NCBI ID   WP_086774524.1    Uniprot ID   -
Organism   Vibrio coralliirubri strain DSM 27495     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269448..280059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV20_RS01215 - 270134..272695 (-) 2562 WP_202910129.1 penicillin-binding protein 1A -
  OCV20_RS01220 pilM 272847..273872 (+) 1026 WP_086774526.1 type IV pilus assembly protein PilM Machinery gene
  OCV20_RS01225 pilN 273856..274455 (+) 600 WP_086774525.1 PilN domain-containing protein Machinery gene
  OCV20_RS01230 pilO 274448..275059 (+) 612 WP_086774524.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV20_RS01235 pilP 275046..275564 (+) 519 WP_086774523.1 pilus assembly protein PilP Machinery gene
  OCV20_RS01240 pilQ 275600..277318 (+) 1719 WP_086774522.1 type IV pilus secretin PilQ Machinery gene
  OCV20_RS01245 aroK 277495..278013 (+) 519 WP_017061125.1 shikimate kinase AroK -
  OCV20_RS01250 aroB 278039..279127 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23552.02 Da        Isoelectric Point: 4.5843

>NTDB_id=91771 OCV20_RS01230 WP_086774524.1 274448..275059(+) (pilO) [Vibrio coralliirubri strain DSM 27495]
MANLQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDEELKQMKQQEQTLKTTLRIKANKVAALPKL
QNQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNALTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGDFSAAIA
KLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=91771 OCV20_RS01230 WP_086774524.1 274448..275059(+) (pilO) [Vibrio coralliirubri strain DSM 27495]
ATGGCTAACCTTCAAAATAGGATGAGCTTGCAAGATCTCGATGTTGATGAGATCACCGAATGGCCATTATTGCCTCAGCT
TCTCGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTTGGCACTTGGCTTTATGTGCTGCCGCTCGATGAGGAGCTGA
AACAGATGAAGCAACAGGAGCAGACCTTAAAAACGACCTTGAGAATAAAGGCTAATAAGGTTGCAGCCTTGCCTAAACTT
CAGAACCAATTAGATGAGTTAACCAGCCGTTACGATTACTTGTTAGAGCAGCTACCTGTTCAAAAGGAGTTGGCGAGTAT
GTTGGCCTCAGTGAATGAGCTCGGCTTGGATAACGCGCTGACGTTTACGCGAATTGATTGGGGGCAAAAGCAGAACAAGG
AGTTCCTTTATCGATTACCTCTCAATATTGAGCTGACAGGTGACTATCACGAGATTGGGGACTTCTCTGCTGCCATCGCC
AAGCTGCCGCGCATTATCAGCTTTGATGATGTGAATTGGCAGCGAGTCAGCCAAGAAAGTAGCACGCTGCACTTTAGGGT
TCGAGCTTATACCTACCAGTTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.13

95.074

0.714
  pilO Vibrio campbellii strain DS40M4

72.432

91.133

0.66

Multiple sequence alignment