Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV20_RS01220 Genome accession   NZ_AP025470
Coordinates   272847..273872 (+) Length   341 a.a.
NCBI ID   WP_086774526.1    Uniprot ID   -
Organism   Vibrio coralliirubri strain DSM 27495     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 267847..278872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV20_RS01205 - 268172..268900 (-) 729 WP_017061133.1 glutathione peroxidase -
  OCV20_RS25865 - 268935..269130 (-) 196 Protein_205 hypothetical protein -
  OCV20_RS01210 oxyR 269129..270022 (+) 894 WP_017061132.1 DNA-binding transcriptional regulator OxyR -
  OCV20_RS01215 - 270134..272695 (-) 2562 WP_202910129.1 penicillin-binding protein 1A -
  OCV20_RS01220 pilM 272847..273872 (+) 1026 WP_086774526.1 type IV pilus assembly protein PilM Machinery gene
  OCV20_RS01225 pilN 273856..274455 (+) 600 WP_086774525.1 PilN domain-containing protein Machinery gene
  OCV20_RS01230 pilO 274448..275059 (+) 612 WP_086774524.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV20_RS01235 pilP 275046..275564 (+) 519 WP_086774523.1 pilus assembly protein PilP Machinery gene
  OCV20_RS01240 pilQ 275600..277318 (+) 1719 WP_086774522.1 type IV pilus secretin PilQ Machinery gene
  OCV20_RS01245 aroK 277495..278013 (+) 519 WP_017061125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38281.73 Da        Isoelectric Point: 6.4182

>NTDB_id=91769 OCV20_RS01220 WP_086774526.1 272847..273872(+) (pilM) [Vibrio coralliirubri strain DSM 27495]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPDKKNWLLVDVGIDQTSLGVIPQNSAPFYKDIAFGTQDLRSSDDPSDIESVFGTIEETH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGVSIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=91769 OCV20_RS01220 WP_086774526.1 272847..273872(+) (pilM) [Vibrio coralliirubri strain DSM 27495]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCTGTGGTCCTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTTGCCATTTCGGTACCTGATAACACA
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCTATCTATCAAACCTTCGCCCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAGGAAGTGGTGGATAGCCGAGCGGATGCATTAACTAAGGCTGGCTTTAAACCT
GTGGTGGTGGATACACAGGCGCATGGGTTACTTAATATCTGGCAATTGGCCTCGCGTCTGTATCCCGATAAGAAAAACTG
GTTGCTGGTGGATGTTGGAATCGACCAAACCTCGCTAGGGGTTATCCCGCAGAATTCGGCGCCTTTCTATAAAGACATCG
CCTTTGGGACTCAAGATCTTCGCAGCTCAGATGACCCGAGTGATATCGAGAGCGTGTTCGGTACTATTGAAGAGACACAT
CAATTCATCGTTAACCTGATTGAGAAGCTCAAGCGTCAATTGCAGCTCTACTCCTCGGGAAATGCGCAGCAACCTATCTC
GGGGATCTGGCTGATGGGCGAGGGCGTCAGCATTCCTATGGTAACCGAAGAGCTAGAACGTCATTTTCAGCTGAGCTGTG
AGTCATTGAATCCTTTGTCTCTGTTTGAGAATAAGGTCGCCAAGAGACGCCGACTGCCGATGGACTGGCAACACTTTGGT
ATCGCGGCAGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.545

100

0.545
  pilM Vibrio campbellii strain DS40M4

48.105

100

0.484

Multiple sequence alignment