Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OCV20_RS01225 Genome accession   NZ_AP025470
Coordinates   273856..274455 (+) Length   199 a.a.
NCBI ID   WP_086774525.1    Uniprot ID   -
Organism   Vibrio coralliirubri strain DSM 27495     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268856..279455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV20_RS25865 - 268935..269130 (-) 196 Protein_205 hypothetical protein -
  OCV20_RS01210 oxyR 269129..270022 (+) 894 WP_017061132.1 DNA-binding transcriptional regulator OxyR -
  OCV20_RS01215 - 270134..272695 (-) 2562 WP_202910129.1 penicillin-binding protein 1A -
  OCV20_RS01220 pilM 272847..273872 (+) 1026 WP_086774526.1 type IV pilus assembly protein PilM Machinery gene
  OCV20_RS01225 pilN 273856..274455 (+) 600 WP_086774525.1 PilN domain-containing protein Machinery gene
  OCV20_RS01230 pilO 274448..275059 (+) 612 WP_086774524.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV20_RS01235 pilP 275046..275564 (+) 519 WP_086774523.1 pilus assembly protein PilP Machinery gene
  OCV20_RS01240 pilQ 275600..277318 (+) 1719 WP_086774522.1 type IV pilus secretin PilQ Machinery gene
  OCV20_RS01245 aroK 277495..278013 (+) 519 WP_017061125.1 shikimate kinase AroK -
  OCV20_RS01250 aroB 278039..279127 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22957.41 Da        Isoelectric Point: 9.6562

>NTDB_id=91770 OCV20_RS01225 WP_086774525.1 273856..274455(+) (pilN) [Vibrio coralliirubri strain DSM 27495]
MLHQINLLPWRDEIRAQHKKRFIHLVILGVIIALIGQWAVGHYFHDQQAKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLDNTTASRKDKGAHHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=91770 OCV20_RS01225 WP_086774525.1 273856..274455(+) (pilN) [Vibrio coralliirubri strain DSM 27495]
ATGTTGCATCAAATTAACCTGCTGCCGTGGCGTGATGAGATTCGTGCTCAGCACAAGAAGCGTTTTATCCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTATAGGGCAATGGGCGGTTGGTCACTATTTTCACGACCAACAAGCCAAGCAGCAAGCTC
GCCTCAATTACCTCAATCAGTACATTGCTGAGCTCGACCGACAAATTCAGTCGTTAAAAGTCGCAGAGCAAGAACACAAA
GCCATTCTTACTCGACTTGATGTGGTTGAGTCGCTGCAGCTTGGCCGCAATAAGACCACCGACTTTATGAACTTAATGCC
GGAGCTGATTCCAGAGGGGGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATTAGCGACA
GTACTGCTCGTCTCGCCACCATGTTGGACAACCTCGAGCGTTCAGATTCTCTGAAAGGGGTCGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTCAATAAAGAATTTCAGACCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTGTCTTTAGACAACAC
AACCGCGAGCAGAAAAGATAAGGGGGCGCATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.5

100

0.638
  pilN Vibrio campbellii strain DS40M4

64.13

92.462

0.593

Multiple sequence alignment