NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	U2H13_RS12615	Genome accession	NZ_CP141181
Coordinates	2590933..2591547 (-)	Length	204 a.a.
NCBI ID	WP_376700480.1	Uniprot ID	-
Organism	Bacillus sp. A1
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2585933..2596547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U2H13_RS12585 (U2H13_12570)	rpsT	2586236..2586502 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
U2H13_RS12590 (U2H13_12575)	holA	2586465..2587560 (-)	1096	Protein_2433	DNA polymerase III subunit delta	-
U2H13_RS12595 (U2H13_12580)	-	2587600..2587751 (-)	152	Protein_2434	hypothetical protein	-
U2H13_RS12600 (U2H13_12585)	-	2587792..2587926 (+)	135	WP_003152870.1	YqzM family protein	-
U2H13_RS12605 (U2H13_12590)	comEC	2587945..2590296 (-)	2352	WP_376700479.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
U2H13_RS12610 (U2H13_12595)	-	2590297..2590866 (-)	570	WP_003152868.1	ComE operon protein 2	-
U2H13_RS12615 (U2H13_12600)	comEA	2590933..2591547 (-)	615	WP_376700480.1	helix-hairpin-helix domain-containing protein	Machinery gene
U2H13_RS12620 (U2H13_12605)	comER	2591606..2592427 (+)	822	WP_015240246.1	late competence protein ComER	-
U2H13_RS12625 (U2H13_12610)	-	2592496..2593233 (-)	738	WP_167408922.1	class I SAM-dependent methyltransferase	-
U2H13_RS12630 (U2H13_12615)	rsfS	2593230..2593586 (-)	357	WP_003152864.1	ribosome silencing factor	-
U2H13_RS12635 (U2H13_12620)	yqeK	2593583..2594164 (-)	582	WP_376700481.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
U2H13_RS12640 (U2H13_12625)	-	2594154..2594723 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
U2H13_RS12645 (U2H13_12630)	yhbY	2594734..2595024 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
U2H13_RS12650 (U2H13_12635)	aroE	2595018..2595860 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22253.37 Da Isoelectric Point: 5.5211

                                >NTDB_id=916261 U2H13_RS12615 WP_376700480.1 2590933..2591547(-) (comEA) [Bacillus sp. A1]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTVSASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=916261 U2H13_RS12615 WP_376700480.1 2590933..2591547(-) (comEA) [Bacillus sp. A1]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGTCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363

Detailed information NTG0916261