NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	S0I03_RS12690	Genome accession	NZ_CP140613
Coordinates	2585674..2586288 (-)	Length	204 a.a.
NCBI ID	WP_014418419.1	Uniprot ID	I2C7Y9
Organism	Bacillus velezensis strain WN-I
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2580674..2591288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S0I03_RS12660	rpsT	2580977..2581243 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
S0I03_RS12665	holA	2581260..2582301 (-)	1042	Protein_2447	DNA polymerase III subunit delta	-
S0I03_RS12670	-	2582341..2582492 (-)	152	Protein_2448	hypothetical protein	-
S0I03_RS12675	-	2582533..2582667 (+)	135	WP_003152870.1	YqzM family protein	-
S0I03_RS12680	comEC	2582686..2585037 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
S0I03_RS12685	-	2585038..2585607 (-)	570	WP_003152868.1	ComE operon protein 2	-
S0I03_RS12690	comEA	2585674..2586288 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
S0I03_RS12695	comER	2586347..2587168 (+)	822	WP_014418420.1	late competence protein ComER	-
S0I03_RS12700	-	2587237..2587974 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
S0I03_RS12705	rsfS	2587971..2588327 (-)	357	WP_007408260.1	ribosome silencing factor	-
S0I03_RS12710	yqeK	2588345..2588905 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
S0I03_RS12715	-	2588895..2589464 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
S0I03_RS12720	yhbY	2589475..2589765 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
S0I03_RS12725	aroE	2589759..2590601 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22226.26 Da Isoelectric Point: 5.0355

                                >NTDB_id=914850 S0I03_RS12690 WP_014418419.1 2585674..2586288(-) (comEA) [Bacillus velezensis strain WN-I]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=914850 S0I03_RS12690 WP_014418419.1 2585674..2586288(-) (comEA) [Bacillus velezensis strain WN-I]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT

GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	I2C7Y9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363

Detailed information NTG0914850