Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AABK05_RS01740 Genome accession   NZ_AP027877
Coordinates   385735..386973 (+) Length   412 a.a.
NCBI ID   WP_088375911.1    Uniprot ID   -
Organism   Escherichia coli strain 2018-05-2CC     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380735..391973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABK05_RS01715 (VEE46_03350) mrcA 381015..383567 (-) 2553 WP_001400412.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  AABK05_RS01720 (VEE46_03360) hofM 383687..384466 (+) 780 WP_001308095.1 DNA utilization protein HofM -
  AABK05_RS01725 (VEE46_03370) hofN 384466..385005 (+) 540 WP_338388022.1 DNA utilization protein HofN -
  AABK05_RS01730 (VEE46_03380) hofO 384989..385429 (+) 441 WP_338388023.1 DNA utilization protein HofO -
  AABK05_RS01735 (VEE46_03390) hofP 385419..385823 (+) 405 WP_028132028.1 DNA utilization protein HofP -
  AABK05_RS01740 (VEE46_03400) pilQ 385735..386973 (+) 1239 WP_088375911.1 DNA uptake porin HofQ Machinery gene
  AABK05_RS01745 (VEE46_03410) aroK 387374..387895 (+) 522 WP_000818618.1 shikimate kinase AroK -
  AABK05_RS01750 (VEE46_03420) aroB 387952..389040 (+) 1089 WP_000439848.1 3-dehydroquinate synthase -
  AABK05_RS01755 (VEE46_03430) damX 389132..390418 (+) 1287 WP_000343210.1 cell division protein DamX -
  AABK05_RS01760 (VEE46_03440) dam 390525..391361 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44775.34 Da        Isoelectric Point: 5.9488

>NTDB_id=91327 AABK05_RS01740 WP_088375911.1 385735..386973(+) (pilQ) [Escherichia coli strain 2018-05-2CC]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQVRAQANLPLENRNIILQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=91327 AABK05_RS01740 WP_088375911.1 385735..386973(+) (pilQ) [Escherichia coli strain 2018-05-2CC]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGCCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAAGCGGA
GCAGGTGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTATTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCTGGGGAAAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCACTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAAGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTGCCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

38.208

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362

Multiple sequence alignment