Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SOJ49_RS18600 Genome accession   NZ_CP139482
Coordinates   4110186..4111301 (-) Length   371 a.a.
NCBI ID   WP_369855979.1    Uniprot ID   -
Organism   Candidatus Thalassolituus haligoni strain BB40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4105186..4116301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOJ49_RS18565 - 4106133..4106750 (+) 618 WP_369855973.1 molybdenum cofactor guanylyltransferase -
  SOJ49_RS18570 moaB 4106756..4107301 (+) 546 WP_369855974.1 molybdenum cofactor biosynthesis protein B -
  SOJ49_RS18575 moaC 4107446..4107904 (+) 459 WP_369858087.1 cyclic pyranopterin monophosphate synthase MoaC -
  SOJ49_RS18580 - 4107901..4108152 (+) 252 WP_369855975.1 MoaD/ThiS family protein -
  SOJ49_RS18585 moaE 4108155..4108652 (+) 498 WP_369855976.1 molybdopterin synthase catalytic subunit MoaE -
  SOJ49_RS18590 - 4108729..4109190 (+) 462 WP_369855977.1 isoprenylcysteine carboxylmethyltransferase family protein -
  SOJ49_RS18595 - 4109266..4110078 (+) 813 WP_369855978.1 class III extradiol ring-cleavage dioxygenase -
  SOJ49_RS18600 pilU 4110186..4111301 (-) 1116 WP_369855979.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SOJ49_RS18605 pilT 4111336..4112370 (-) 1035 WP_369855980.1 type IV pilus twitching motility protein PilT Machinery gene
  SOJ49_RS18610 - 4112519..4113223 (+) 705 WP_369855981.1 YggS family pyridoxal phosphate-dependent enzyme -
  SOJ49_RS18615 proC 4113257..4114075 (+) 819 WP_369855982.1 pyrroline-5-carboxylate reductase -
  SOJ49_RS18620 - 4114133..4114723 (+) 591 WP_369855983.1 YggT family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40981.30 Da        Isoelectric Point: 6.8847

>NTDB_id=909967 SOJ49_RS18600 WP_369855979.1 4110186..4111301(-) (pilU) [Candidatus Thalassolituus haligoni strain BB40]
MSMDKFLRLMVDKGASDLFITAGVAPSMKVNGQILPLGKSPLAAEQTRDIVLGLMNSKQQDEFESTKELNFAINARGIGR
FRASAFYQRNVAGMVLRRIETRIPGVDQLGLPDIIKELAMTKRGLILFVGATGTGKSTSLAAMIGHRNRNSKGHILSIED
PIEYIHQHDGCIITQREVGLDTASFEVALKNCLRQAPDVIMIGEVRSPDIMESAVTFAETGHLCLATLHANNANQALERV
IHFFPPERHGQVWMDLSLNLKAIVAQQLIPTPDGRSRRAVIEVLINSPLASDLIRKGDVHALKPLMKKSNEMGMQTFDQA
LYQLYEQGEITYEDALAHADSPNDLRLMIKLASETDAEHLVGMSKSFKLEN

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=909967 SOJ49_RS18600 WP_369855979.1 4110186..4111301(-) (pilU) [Candidatus Thalassolituus haligoni strain BB40]
ATGTCGATGGACAAGTTTCTACGATTGATGGTCGATAAAGGCGCATCGGATCTGTTTATTACAGCCGGTGTAGCGCCATC
AATGAAGGTGAATGGCCAGATTTTACCGCTGGGTAAATCGCCGCTGGCGGCGGAGCAAACGCGGGATATCGTGCTCGGGT
TGATGAACAGCAAGCAGCAGGATGAGTTTGAAAGCACCAAGGAACTGAATTTTGCCATTAATGCCCGAGGCATCGGGCGT
TTTCGCGCCAGTGCGTTTTATCAGCGTAACGTCGCGGGCATGGTGCTGCGGCGAATCGAGACCCGGATTCCGGGTGTTGA
CCAGCTTGGCTTGCCCGATATCATCAAGGAACTGGCGATGACCAAGCGTGGCCTGATCCTGTTTGTCGGCGCGACAGGAA
CGGGCAAATCGACGTCGCTGGCGGCGATGATTGGTCATCGTAATCGTAACAGCAAGGGCCATATTCTCAGCATTGAAGAC
CCGATTGAATATATCCATCAGCATGACGGCTGCATCATCACCCAGCGTGAGGTCGGCCTTGATACCGCCAGTTTTGAGGT
GGCGCTGAAGAACTGTCTGCGTCAGGCACCGGATGTCATCATGATTGGCGAGGTACGTTCACCGGATATTATGGAGAGTG
CAGTGACCTTTGCCGAGACGGGTCACTTGTGTCTGGCAACGTTACACGCCAACAACGCCAACCAGGCGCTGGAACGGGTG
ATTCACTTTTTTCCGCCGGAACGGCATGGCCAGGTCTGGATGGATTTATCGCTTAATCTGAAGGCGATTGTTGCCCAGCA
ACTGATACCGACGCCGGATGGCCGTTCGCGTCGGGCGGTGATTGAAGTACTGATCAACTCACCACTGGCATCGGATCTGA
TTCGTAAGGGTGATGTACACGCGCTCAAGCCATTGATGAAAAAATCCAACGAGATGGGGATGCAGACCTTTGATCAGGCC
TTGTATCAATTGTATGAGCAAGGCGAAATCACCTATGAAGATGCGCTGGCGCATGCGGATTCACCCAACGATCTGCGACT
GATGATCAAGCTGGCATCGGAAACCGATGCGGAGCATCTGGTTGGCATGAGCAAGAGTTTCAAACTCGAAAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

74.865

99.73

0.747
  pilU Acinetobacter baylyi ADP1

63.305

96.226

0.609
  pilU Vibrio cholerae strain A1552

56.286

94.34

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.816

93.801

0.402