Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   L6431_RS05795 Genome accession   NZ_AP025273
Coordinates   1280672..1281736 (+) Length   354 a.a.
NCBI ID   WP_021698834.1    Uniprot ID   U2ZGY5
Organism   Aquipseudomonas alcaligenes strain MRCP2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1275672..1286736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6431_RS05785 - 1276743..1278011 (+) 1269 WP_061903204.1 malic enzyme-like NAD(P)-binding protein -
  L6431_RS05790 - 1278073..1280487 (-) 2415 WP_373869750.1 penicillin-binding protein 1A -
  L6431_RS05795 pilM 1280672..1281736 (+) 1065 WP_021698834.1 pilus assembly protein PilM Machinery gene
  L6431_RS05800 - 1281736..1282362 (+) 627 WP_021698833.1 PilN domain-containing protein -
  L6431_RS05805 pilO 1282359..1282991 (+) 633 WP_237045988.1 type 4a pilus biogenesis protein PilO -
  L6431_RS05810 pilP 1282988..1283515 (+) 528 WP_021698831.1 type 4a pilus biogenesis lipoprotein PilP -
  L6431_RS05815 pilQ 1283568..1285640 (+) 2073 WP_043216064.1 type IV pilus secretin PilQ Machinery gene
  L6431_RS05820 aroK 1285643..1286161 (+) 519 WP_021698829.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37951.34 Da        Isoelectric Point: 4.4552

>NTDB_id=90904 L6431_RS05795 WP_021698834.1 1280672..1281736(+) (pilM) [Aquipseudomonas alcaligenes strain MRCP2]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLSKAKTSVRTAAV
AVAGSAVITKTIEMEAGLNDDELENQLKIEADQYIPYPLEEVAIDFEVQGLSPRNPERVEVLLAACRKENVEVREAALGL
AGVTAKVVDVEAYALERAYGLLQSQLGGSVDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRMIQQKIGTPTLVANP
FADMALGSKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=90904 L6431_RS05795 WP_021698834.1 1280672..1281736(+) (pilM) [Aquipseudomonas alcaligenes strain MRCP2]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTTCTGGGGATCGACATCAGTTCGACCTCGGTCAAGCTCCTTGAATT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCCTATGCTGTCGAGCCGCTCCCACCCAATGCTGTTGTCGAAAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCTCTTGCTCGCGTTCTGAGCAAGGCGAAAACCAGCGTGCGTACTGCGGCAGTG
GCGGTGGCCGGATCTGCGGTGATCACTAAGACCATCGAGATGGAAGCCGGCCTCAACGATGACGAGCTGGAGAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCATTGGAAGAGGTGGCTATCGACTTCGAGGTGCAGGGCCTTTCTCCGC
GCAACCCCGAACGAGTCGAAGTCCTGCTGGCTGCCTGCCGTAAAGAGAACGTCGAGGTTCGCGAGGCGGCTCTTGGGCTC
GCCGGCGTTACAGCCAAGGTTGTCGATGTGGAGGCTTATGCGCTCGAGCGCGCCTATGGCCTGCTCCAGTCACAGCTTGG
CGGCAGCGTCGATGAGCTGACGGTTGCCGTGGTCGACATCGGTGCCACCATGACCACCCTGAGCGTGCTGCACAATGGTC
GCACGATCTACACCCGGGAGCAGTTGTTCGGTGGGCGTCAGCTGACCGAAGAGATCCAGCGCCGCTACGGTCTCTCCGTC
GAGGAGGCTGGTCTGGCCAAGAAGCAGGGTGGTCTGCCGGATGACTACGAAAGCGAAGTGCTGCAGCCCTTCAAGGAAGC
GGTCGTGCAGCAGGTTTCGCGTTCGCTGCAATTCTTCTTCGCGGCCGGTCAGTTCAACGATGTCGACTACATCCTGCTGG
CAGGTGGTACGGCCTCGATTCCTGACCTGGATCGAATGATTCAACAGAAGATTGGTACTCCTACCCTGGTGGCCAACCCC
TTCGCCGACATGGCGCTGGGAAGCAAGGTCAATGCCGGAGCCCTGGCTAGCGATGCCCCGTCCTTGATGATTGCCTGTGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2ZGY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.458

Multiple sequence alignment