Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L6431_RS05815 Genome accession   NZ_AP025273
Coordinates   1283568..1285640 (+) Length   690 a.a.
NCBI ID   WP_043216064.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain MRCP2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1278568..1290640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6431_RS05795 pilM 1280672..1281736 (+) 1065 WP_021698834.1 pilus assembly protein PilM Machinery gene
  L6431_RS05800 - 1281736..1282362 (+) 627 WP_021698833.1 PilN domain-containing protein -
  L6431_RS05805 pilO 1282359..1282991 (+) 633 WP_237045988.1 type 4a pilus biogenesis protein PilO -
  L6431_RS05810 pilP 1282988..1283515 (+) 528 WP_021698831.1 type 4a pilus biogenesis lipoprotein PilP -
  L6431_RS05815 pilQ 1283568..1285640 (+) 2073 WP_043216064.1 type IV pilus secretin PilQ Machinery gene
  L6431_RS05820 aroK 1285643..1286161 (+) 519 WP_021698829.1 shikimate kinase AroK -
  L6431_RS05825 aroB 1286211..1287314 (+) 1104 WP_021698828.1 3-dehydroquinate synthase -
  L6431_RS05830 - 1287324..1288976 (+) 1653 WP_237045989.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74303.12 Da        Isoelectric Point: 4.6218

>NTDB_id=90905 L6431_RS05815 WP_043216064.1 1283568..1285640(+) (pilQ) [Aquipseudomonas alcaligenes strain MRCP2]
MNSALSRVAISLISGFIASAAVAADLSGLDVAALPGDRVELKLQFDSPVVAPRGYTIEQPARIALDLPGVANKLGVKNRE
LGVGNARSVTIVETKDRTRLIVNLSTLVPYATRAEGNNLFVVVGEAATSASNAVATPAQSLTKSVPVSGRAIQGVDFQRG
EEGEGNVVIALSDSSVTADIQEQGGKIQLSFPKAALPDSLKVRLDVKDFATPVQYVSASAQADKVNISIEPQGFYDYLVF
QADNKLTVSVKPLSSDDVEKRKLERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQAL
DLVLKTRGLDKRKVGNVLLIAPADEIAARERQELEAQNQIAELAPLRRELIQVNYAKASDIAALFQSVAGIGGEGAKEER
GSVTVDDRTNSIIAYQTQDKLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKELGVRWGGSTNLQGDKNWTVYGNDDRG
DEGGRTGATQGPNVPFVDLGAAGRTSGIGIGFVTDNVLLDLELSAMEKTGNGEVISQPKVVTSDKETAKILKGTEVPYEE
SSSSGATTIAFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYQNALANVPPIKKNEVNAKVLVNDGETIVIGGVFSNEQ
SKAVDKVPLLGDMPFVGRLFRRDVVKDTKNELLVFITPRIMNNQSIAVSN

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=90905 L6431_RS05815 WP_043216064.1 1283568..1285640(+) (pilQ) [Aquipseudomonas alcaligenes strain MRCP2]
ATGAATAGCGCCCTCTCTCGCGTTGCCATCTCATTGATTTCAGGGTTTATTGCTTCGGCTGCAGTAGCAGCAGACCTCAG
TGGATTGGATGTGGCTGCGCTCCCTGGTGATAGGGTTGAGCTTAAGTTGCAGTTTGACAGCCCCGTGGTGGCTCCGCGTG
GCTATACGATAGAGCAGCCCGCGCGCATTGCTTTGGATCTGCCCGGTGTGGCAAACAAGCTGGGTGTTAAGAATCGTGAG
CTTGGTGTGGGAAATGCGCGAAGCGTGACCATCGTCGAGACAAAGGATCGGACCCGTCTGATAGTTAACTTGTCAACTCT
GGTCCCCTACGCGACTAGGGCTGAGGGTAACAATCTATTTGTGGTTGTGGGTGAGGCTGCGACTTCAGCTTCCAATGCGG
TTGCCACTCCAGCGCAATCGTTGACTAAATCTGTCCCTGTCTCCGGTCGAGCGATCCAAGGTGTTGATTTTCAACGTGGT
GAGGAAGGCGAAGGGAATGTCGTCATTGCTTTGTCGGACTCCTCGGTGACGGCCGATATTCAGGAGCAAGGCGGGAAAAT
CCAGCTGTCCTTTCCTAAAGCTGCCCTCCCTGACAGCCTCAAGGTTCGTCTCGATGTGAAGGATTTTGCGACGCCTGTTC
AATATGTGAGTGCGTCTGCCCAGGCTGATAAGGTAAATATAAGTATTGAGCCTCAGGGTTTTTACGATTACTTGGTCTTC
CAGGCGGATAACAAGCTGACAGTCAGTGTTAAGCCCCTGTCTTCTGATGATGTCGAGAAGCGTAAGCTTGAGCGTTTTGC
TTACACCGGTGAGAAGCTTTCTCTTAATTTTCAGGATATTGATGTTCGATCTGTGTTACAGCTGATTGCTGACTTCACTG
ATTTGAATCTGGTGGCGAGTGATACTGTTCAGGGTAACATTACCCTGCGGCTGCAAAATGTGCCGTGGGACCAAGCTTTG
GATCTTGTGCTGAAGACGCGTGGCTTGGACAAGAGGAAGGTTGGGAACGTATTGCTTATTGCTCCCGCGGATGAGATAGC
GGCACGTGAGCGTCAGGAGTTGGAGGCGCAAAATCAAATTGCAGAGCTTGCGCCGCTGCGTCGTGAGCTGATTCAGGTGA
ATTATGCTAAGGCATCTGATATTGCAGCTTTGTTCCAATCTGTTGCGGGTATCGGTGGTGAAGGCGCTAAGGAGGAGAGG
GGTTCGGTTACAGTGGATGATCGAACTAACAGTATTATTGCTTATCAAACACAGGATAAATTAGATGAGTTGCGACGTAT
TGTTGCTCAGCTTGATATTCCTGTGCGGCAGGTAATGATTGAGGCGCGAATTGTTGAGGCCAATGTTGACTATGACAAGG
AGCTCGGCGTTCGTTGGGGCGGCAGCACCAATCTGCAGGGTGATAAGAACTGGACTGTCTATGGTAATGACGATCGCGGT
GACGAGGGCGGGCGCACTGGTGCAACCCAAGGGCCCAACGTTCCTTTTGTTGATCTCGGCGCTGCTGGTCGTACCTCTGG
TATCGGTATTGGCTTCGTAACAGACAATGTTTTACTGGATCTCGAGTTGTCTGCTATGGAGAAGACCGGTAACGGTGAGG
TCATCTCGCAACCTAAGGTGGTCACCTCCGATAAGGAAACCGCGAAGATCCTGAAGGGCACTGAAGTTCCCTACGAGGAG
TCCAGTTCTAGCGGTGCTACGACTATTGCATTCAAAGAGGCTGCACTGTCTCTTGAAGTAACTCCGCAGATTACTCCTGA
TAACCGCATCATCATGGAGGTCAAAGTCAACAAGGATGAGCCGGACTATCAGAACGCCCTTGCCAACGTGCCTCCCATCA
AGAAGAACGAGGTCAATGCAAAGGTCCTCGTTAATGATGGTGAGACAATAGTTATCGGCGGCGTCTTCTCGAACGAGCAG
TCCAAGGCTGTTGACAAGGTGCCGCTCTTGGGGGACATGCCTTTTGTCGGTCGCCTGTTCCGTCGTGACGTGGTAAAGGA
TACGAAGAACGAACTGCTGGTCTTCATTACTCCGCGGATCATGAACAATCAGTCAATCGCGGTAAGCAACTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(296-342)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.184

100

0.759
  comQ Acinetobacter baylyi ADP1

41.977

100

0.425
  pilQ Acinetobacter baumannii strain A118

39.646

100

0.422
  pilQ Acinetobacter baumannii D1279779

39.646

100

0.422
  pilQ Legionella pneumophila strain Lp02

39.528

98.261

0.388
  pilQ Legionella pneumophila strain ERS1305867

39.439

98.116

0.387

Multiple sequence alignment