Detailed information NTG0090902

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L6431_RS04970
• Genome accession: NZ_AP025273
• Coordinates: 1094966..1096111 (+)
• Length: 381 a.a.
• NCBI ID: WP_061903063.1
• Uniprot ID: A0AA37CEW7
• Organism: Aquipseudomonas alcaligenes strain MRCP2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1089966..1101111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6431_RS04945	-	1091376..1091672 (-)	297	WP_061903059.1	DUF167 domain-containing protein	-
L6431_RS04950	-	1091674..1092267 (-)	594	WP_021699009.1	YggT family protein	-
L6431_RS04955	proC	1092277..1093095 (-)	819	WP_237045897.1	pyrroline-5-carboxylate reductase	-
L6431_RS04960	-	1093109..1093801 (-)	693	WP_237045898.1	YggS family pyridoxal phosphate-dependent enzyme	-
L6431_RS04965	pilT	1093874..1094908 (+)	1035	WP_021699006.1	type IV pilus twitching motility protein PilT	Machinery gene
L6431_RS04970	pilU	1094966..1096111 (+)	1146	WP_061903063.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L6431_RS04975	-	1096065..1096949 (-)	885	WP_237045899.1	TIGR03915 family putative DNA repair protein	-
L6431_RS04980	-	1096949..1098160 (-)	1212	WP_021699003.1	putative DNA modification/repair radical SAM protein	-
L6431_RS04985	-	1098309..1098707 (+)	399	WP_021699002.1	NINE protein	-
L6431_RS04990	-	1098763..1100034 (-)	1272	WP_021699001.1	dihydroorotase	-
L6431_RS04995	-	1100034..1101035 (-)	1002	WP_021699000.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42614.90 Da
• Isoelectric Point: 6.8370

>NTDB_id=90902 L6431_RS04970 WP_061903063.1 1094966..1096111(+) (pilU) [Aquipseudomonas alcaligenes strain MRCP2]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEQTRETVHSVMNEQQRREFAEKHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNTSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLALATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTELGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSMSEGLSLQVSEDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=90902 L6431_RS04970 WP_061903063.1 1094966..1096111(+) (pilU) [Aquipseudomonas alcaligenes strain MRCP2]
ATGGAATTTGAAAAGCTGCTGCGTCTGATGGTGGAGAAGGGTGGTTCCGACCTGTTCATCACTGCCGGCGTGCCTCCTTC
GATGAAGGTCAATGGCAAGATCATGCCGGTGACCAAGAATCCCATGTCGCCGGAGCAGACCCGCGAGACGGTGCACTCGG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCCGAAAAGCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGCCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTCGCTGGCATGGTGCTGCGCCGCATCGAGACCAATATCCCGACCCTCGA
TGACCTCAAGCTGCCCGAGGTGCTGAAGAAGCTGGCGCTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACACCAGCGGCCATATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTCGGTATCGATACCGAATCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATCGGCGAGATCCGTACCCGCGAGACCATGGACTATG
CCGTGGCCTTCGCCGAAACCGGCCACCTGGCCCTGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTGTGGATGGATCTGTCGCTTAACCTCAAGGCGATCGTCGCCCAGCA
ACTGGTGCCAACCCCGGACGGCAAGGGCCGGCGTGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TTCGCAAGGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACAGTCAGGGCGAGATCACCTACGAGGACGCCCTGCTGTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGTTCGGAGACCGACGGCGAGCACCTCACCAGCATGAGCGAAGGCCTCAGCCTGCAGGTGAGCGAGG
ACGATCCGGGCCGGCGTTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.388	100	0.924
pilU	Acinetobacter baylyi ADP1	64.986	93.701	0.609
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.452	88.189	0.383