Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   SOI69_RS16805 Genome accession   NZ_CP139248
Coordinates   3570833..3571585 (+) Length   250 a.a.
NCBI ID   WP_060464993.1    Uniprot ID   A0A1E2YH36
Organism   Acinetobacter pittii strain HCG18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3565833..3576585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI69_RS16790 (SOI69_16790) ponA 3566414..3568975 (-) 2562 WP_320539673.1 penicillin-binding protein PBP1a -
  SOI69_RS16795 (SOI69_16795) pilM 3569137..3570195 (+) 1059 WP_320539674.1 pilus assembly protein PilM Machinery gene
  SOI69_RS16800 (SOI69_16800) pilN 3570195..3570836 (+) 642 WP_005067080.1 PilN domain-containing protein Machinery gene
  SOI69_RS16805 (SOI69_16805) pilO 3570833..3571585 (+) 753 WP_060464993.1 type 4a pilus biogenesis protein PilO Machinery gene
  SOI69_RS16810 (SOI69_16810) pilP 3571596..3572123 (+) 528 WP_068563956.1 pilus assembly protein PilP Machinery gene
  SOI69_RS16815 (SOI69_16815) pilQ 3572186..3574336 (+) 2151 WP_320539675.1 type IV pilus secretin PilQ Machinery gene
  SOI69_RS16820 (SOI69_16820) aroK 3574348..3574917 (+) 570 WP_320539676.1 shikimate kinase AroK -
  SOI69_RS16825 (SOI69_16825) aroB 3574946..3576028 (+) 1083 WP_017481728.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28241.10 Da        Isoelectric Point: 4.8782

>NTDB_id=908260 SOI69_RS16805 WP_060464993.1 3570833..3571585(+) (pilO) [Acinetobacter pittii strain HCG18]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=908260 SOI69_RS16805 WP_060464993.1 3570833..3571585(+) (pilO) [Acinetobacter pittii strain HCG18]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGTAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGGTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACTGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E2YH36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

69.583

96

0.668