Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ABK819_RS28785 Genome accession   NZ_CP157460
Coordinates   6159074..6159697 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6154074..6164697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK819_RS28765 (ABK819_28740) aroB 6154669..6155775 (-) 1107 WP_019484454.1 3-dehydroquinate synthase -
  ABK819_RS28770 (ABK819_28745) aroK 6155825..6156343 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ABK819_RS28775 (ABK819_28750) pilQ 6156355..6158499 (-) 2145 WP_015503770.1 type 4a pilus secretin PilQ Machinery gene
  ABK819_RS28780 (ABK819_28755) comL/pilP 6158553..6159077 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  ABK819_RS28785 (ABK819_28760) pilO 6159074..6159697 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABK819_RS28790 (ABK819_28765) pilN 6159694..6160290 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  ABK819_RS28795 (ABK819_28770) pilM 6160290..6161354 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  ABK819_RS28800 (ABK819_28775) - 6161539..6164010 (+) 2472 WP_019484455.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=905745 ABK819_RS28785 WP_003103268.1 6159074..6159697(-) (pilO) [Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=905745 ABK819_RS28785 WP_003103268.1 6159074..6159697(-) (pilO) [Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ]
ATGAGTCTGGCCAGTTCCCTGGAAAGTTTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401