Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RZE77_RS02370 Genome accession   NZ_CP138513
Coordinates   529363..530955 (+) Length   530 a.a.
NCBI ID   WP_319440529.1    Uniprot ID   -
Organism   Variovorax paradoxus strain SPNA7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524363..535955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE77_RS02345 (RZE77_02345) argS 524476..526161 (+) 1686 WP_319440525.1 arginine--tRNA ligase -
  RZE77_RS02350 (RZE77_02350) - 526169..526894 (+) 726 WP_319440526.1 SPOR domain-containing protein -
  RZE77_RS02355 (RZE77_02355) - 527039..527686 (+) 648 WP_319440527.1 thiol:disulfide interchange protein DsbA/DsbL -
  RZE77_RS02360 (RZE77_02360) lptA 527835..528554 (+) 720 WP_319440528.1 lipopolysaccharide transport periplasmic protein LptA -
  RZE77_RS02365 (RZE77_02365) lptB 528551..529345 (+) 795 WP_307656391.1 LPS export ABC transporter ATP-binding protein -
  RZE77_RS02370 (RZE77_02370) rpoN/rpoN1 529363..530955 (+) 1593 WP_319440529.1 RNA polymerase factor sigma-54 Machinery gene
  RZE77_RS02375 (RZE77_02375) - 530966..532570 (+) 1605 WP_319440530.1 phospholipase D family protein -
  RZE77_RS02380 (RZE77_02380) - 532581..533225 (-) 645 WP_319440531.1 LysE family translocator -
  RZE77_RS02385 (RZE77_02385) - 533230..533865 (-) 636 WP_319440532.1 YceI family protein -
  RZE77_RS02390 (RZE77_02390) - 533906..534478 (-) 573 WP_319440533.1 YceI family protein -
  RZE77_RS02395 (RZE77_02395) - 534475..535104 (-) 630 WP_319442288.1 cytochrome b -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58499.78 Da        Isoelectric Point: 4.7226

>NTDB_id=903261 RZE77_RS02370 WP_319440529.1 529363..530955(+) (rpoN/rpoN1) [Variovorax paradoxus strain SPNA7]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAIDTPVPRDEAGEAAEGE
FASAPASSTTSTSSETASTAESDVPAADATEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALSLRLNENDSAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDELVHHFQVALGLLQSLEPAGVGARDL
GECLCIQLRALASESETEEQALVRKTAIAICKQQMELLARRDFKRLATLTRTNEELVRSALQVISRLEPKPGRRFVDVER
NIVIPDVIVTKVGRGTNAKFRVMLNPEVMPRLRVHDIYAGALKSHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=903261 RZE77_RS02370 WP_319440529.1 529363..530955(+) (rpoN/rpoN1) [Variovorax paradoxus strain SPNA7]
ATGAAGCAAGGGCTGTCCCTTCGCGTTTCGCAGCACCTGGCGCTCACGCCCCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTCGAACTGAGCCAGGAAGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CCGCGCGCGAGGAATTCGGGCTCGATGCCATCGACACGCCGGTGCCGCGCGACGAGGCCGGTGAAGCCGCCGAGGGCGAA
TTCGCCTCGGCCCCGGCCAGCAGCACGACCAGCACGTCCAGCGAAACCGCCTCCACGGCCGAGTCCGATGTTCCCGCCGC
CGATGCCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCGCCCGACGACAGCGAATGGGGCAGCG
ATGCCCCCGCGCGCCAGAACAACCTGGGCGACGACGAGCGCGCCGACGCCACCGAGCTCGCGCGCAGCCAGGAGTCGCTG
CAGTCCTTCCTGCACCGGCAGGCGCTGAGCCTGCGGCTCAACGAGAACGACAGCGCCGCGCTGCGCTTCCTGATCGAATC
GCTGAACGACGACGGCTACCTGGAGGATTCGCTGCCGGCGCTGGCCTCGGGCCTGGCGGGCGACGACAACGACCAGTTCG
ACGAGCTGGTGCACCACTTCCAGGTGGCGCTCGGCCTGCTGCAGAGCCTGGAGCCGGCCGGCGTGGGCGCGCGCGACCTG
GGCGAATGCCTTTGCATCCAGCTGCGCGCACTCGCGAGCGAAAGCGAGACCGAGGAACAGGCGCTGGTGCGCAAGACCGC
CATCGCCATCTGCAAGCAGCAGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACCAACG
AAGAGCTGGTGCGCTCGGCGCTGCAGGTCATCTCGCGCCTGGAGCCCAAGCCGGGCCGCCGTTTTGTCGACGTCGAGCGC
AACATCGTCATTCCCGACGTGATCGTCACCAAGGTCGGCCGCGGCACCAATGCCAAGTTCCGCGTCATGCTCAACCCCGA
GGTGATGCCGCGGCTGCGCGTGCACGACATCTATGCCGGCGCGCTCAAGTCGCACAAGGGCGAGGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAGCGGCAGAAGAGCTTCTTCGTGCACGGCGAGCTCGCGATGCGGCCGCTGGTGCTGCGCGAGATTGCCGACGA
ACTCGGCCTGCACGAATCGACCATCTCGCGCGTGACCACGGCCAAGTACATGTCCACGCCTTTCGGCACCGTCGAGCTCA
AGTACTTCTTCGGCTCGGCGCTCGGCACCGAGACCGGCGGCAATGCGTCGAGCACCGCGGTGCGCGCGCTGATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATCTCCGAGATGCTCAAGGAGCAGGGCATCGAGTG
CGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCCCTGCGCATCGCGCCGGCCAACCTTCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.789

100

0.519