Detailed information NTG0902492

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SE337_RS15000
• Genome accession: NZ_CP138374
• Coordinates: 3185833..3187002 (+)
• Length: 389 a.a.
• NCBI ID: WP_054077664.1
• Uniprot ID: -
• Organism: Pseudomonas amygdali pv. sesami strain DFR141
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3180833..3192002

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SE337_RS14985 (SE337_14915)	-	3181270..3183597 (+)	2328	WP_054077662.1	ATP-dependent DNA helicase	-
SE337_RS14990 (SE337_14920)	pgm	3183713..3185359 (+)	1647	WP_054077663.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
SE337_RS14995 (SE337_14925)	-	3185434..3185709 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
SE337_RS15000 (SE337_14930)	pilU	3185833..3187002 (+)	1170	WP_054077664.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SE337_RS15005 (SE337_14935)	-	3187053..3188630 (-)	1578	WP_054077665.1	ABC transporter ATP-binding protein	-
SE337_RS15010 (SE337_14940)	-	3188632..3189654 (-)	1023	WP_054077666.1	ABC transporter permease	-
SE337_RS15015 (SE337_14945)	-	3189654..3190718 (-)	1065	WP_122302425.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42610.74 Da
• Isoelectric Point: 5.8676

>NTDB_id=902492 SE337_RS15000 WP_054077664.1 3185833..3187002(+) (pilU) [Pseudomonas amygdali pv. sesami strain DFR141]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVGTLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=902492 SE337_RS15000 WP_054077664.1 3185833..3187002(+) (pilU) [Pseudomonas amygdali pv. sesami strain DFR141]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTCTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTTGCGGTGATTGCCCAAGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGACGTGATCATGGAAAAGCACGGTCTGGTGTTGTTCGTCGGTGCAACGGGGT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTTGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTA
TTCAATCTGGCCGTCGAAGGTGCGATCAGTGAAGAAGAGGCCCTCAAGAACGCCGACTCACAAAACAACGTGCGCCTGCG
CCTCAAGCTGCACAGTGAAGGCGGCGTCGGAACACTGACCACACCGCCGCCGGCTCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGATGACGAGCCGGGTCCGCAAGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362