Detailed information NTG0901452

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R6238_RS26045
• Genome accession: NZ_CP138212
• Coordinates: 6022908..6024077 (+)
• Length: 389 a.a.
• NCBI ID: WP_010408289.1
• Uniprot ID: A0AB38BZ52
• Organism: Pseudomonas syringae pv. syringae strain pss-2019
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6017908..6029077

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6238_RS26030	-	6018298..6020625 (+)	2328	WP_024960801.1	ATP-dependent DNA helicase	-
R6238_RS26035	pgm	6020789..6022435 (+)	1647	WP_024960802.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
R6238_RS26040	-	6022509..6022784 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
R6238_RS26045	pilU	6022908..6024077 (+)	1170	WP_010408289.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R6238_RS26050	-	6024128..6025705 (-)	1578	WP_003410863.1	ABC transporter ATP-binding protein	-
R6238_RS26055	-	6025707..6026729 (-)	1023	WP_003318547.1	ABC transporter permease	-
R6238_RS26060	-	6026729..6027790 (-)	1062	WP_024960803.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42605.70 Da
• Isoelectric Point: 6.0641

>NTDB_id=901452 R6238_RS26045 WP_010408289.1 6022908..6024077(+) (pilU) [Pseudomonas syringae pv. syringae strain pss-2019]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGTLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=901452 R6238_RS26045 WP_010408289.1 6022908..6024077(+) (pilU) [Pseudomonas syringae pv. syringae strain pss-2019]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTGTACCTCTCCACCGGAGCACCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TTATGGACGAGGAGCAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCTATGGCGGGTATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAGAAGCGCGGTCTGGTGTTGTTTGTCGGGGCCACCGGTT
CCGGGAAGTCGACCTCTTTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGTGGCCATATCATCACCATTGAAGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCACTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCATG
CCCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACACTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGTCTCGGTATGCAGACGTTCGACACGGCGCTA
TTCAATCTGGCCGTTGAAGGTGCAATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGACCCTGACCACACCGCCGCCGGCCCCGACCGGCAGCAGTACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.287	93.059	0.542
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	50.829	93.059	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.903	98.458	0.383
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362