Detailed information NTG0901357

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RA179_RS13100
• Genome accession: NZ_CP138201
• Coordinates: 2566363..2566980 (-)
• Length: 205 a.a.
• NCBI ID: WP_151175157.1
• Uniprot ID: -
• Organism: Bacillus halotolerans strain DY299
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2561363..2571980

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RA179_RS13070 (RA179_13070)	rpsT	2561666..2561932 (+)	267	WP_010335003.1	30S ribosomal protein S20	-
RA179_RS13075 (RA179_13075)	holA	2561947..2562990 (-)	1044	WP_151175155.1	DNA polymerase III subunit delta	-
RA179_RS13080 (RA179_13080)	-	2563030..2563179 (-)	150	WP_151175156.1	hypothetical protein	-
RA179_RS13085 (RA179_13085)	-	2563220..2563354 (+)	135	WP_010335005.1	YqzM family protein	-
RA179_RS13090 (RA179_13090)	comEC	2563393..2565723 (-)	2331	WP_286109244.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RA179_RS13095 (RA179_13095)	-	2565727..2566296 (-)	570	WP_010335007.1	ComE operon protein 2	-
RA179_RS13100 (RA179_13100)	comEA	2566363..2566980 (-)	618	WP_151175157.1	helix-hairpin-helix domain-containing protein	Machinery gene
RA179_RS13105 (RA179_13105)	comER	2567064..2567885 (+)	822	WP_101864554.1	late competence protein ComER	-
RA179_RS13110 (RA179_13110)	-	2567950..2568693 (-)	744	WP_106020124.1	class I SAM-dependent methyltransferase	-
RA179_RS13115 (RA179_13115)	rsfS	2568690..2569046 (-)	357	WP_044154484.1	ribosome silencing factor	-
RA179_RS13120 (RA179_13120)	yqeK	2569064..2569624 (-)	561	WP_106020125.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RA179_RS13125 (RA179_13125)	-	2569614..2570183 (-)	570	WP_010335014.1	nicotinate-nucleotide adenylyltransferase	-
RA179_RS13130 (RA179_13130)	yhbY	2570194..2570484 (-)	291	WP_106020126.1	ribosome assembly RNA-binding protein YhbY	-
RA179_RS13135 (RA179_13135)	aroE	2570484..2571320 (-)	837	WP_106022105.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22045.89 Da
• Isoelectric Point: 4.8695

>NTDB_id=901357 RA179_RS13100 WP_151175157.1 2566363..2566980(-) (comEA) [Bacillus halotolerans strain DY299]
MNWLNQHKTIIMSVGSAAVLIAVLCLFFFSGNNKEPVKQVVSSEKENEEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSKGEEAAQQAAGGAVQSSKGKETLVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=901357 RA179_RS13100 WP_151175157.1 2566363..2566980(-) (comEA) [Bacillus halotolerans strain DY299]
TTGAATTGGTTGAATCAACATAAAACGATAATCATGTCAGTTGGCTCTGCTGCTGTTCTCATTGCAGTTCTTTGCCTCTT
TTTTTTCAGCGGAAATAACAAAGAGCCTGTAAAACAAGTTGTATCTAGTGAAAAGGAAAATGAGGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCGGAAGAAAATATTGTAATCGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTATCGCAGGCTATTGAGAAAGCAGGCGGCATTGGTGAAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTATATACCGAGTAAGGGAGAAGAGGCGGCACAGCAAGCGGCCGGCGGCGCTG
TACAAAGCAGTAAGGGGAAGGAGACATTGGTCAATATCAATACAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGACCTTCAAAAGCCGAAGCGATTATTGCGTATCGGGAAGAGAACGGGCGTTTCCAGACAATCGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (64-119)

• Helix-hairpin-helix motif (HHH_3) (141-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	77.184	100	0.776
comEA	Staphylococcus aureus MW2	37.727	100	0.405
comEA	Staphylococcus aureus N315	37.387	100	0.405
comEA	Streptococcus thermophilus LMD-9	37.264	100	0.385
comEA	Latilactobacillus sakei subsp. sakei 23K	35.78	100	0.38