Detailed information NTG0898686

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5031_RS16140
• Genome accession: NZ_CP137512
• Coordinates: 3466893..3468041 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA0271
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3461893..3473041

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5031_RS16120 (R5031_16120)	-	3462631..3463815 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
R5031_RS16125 (R5031_16125)	-	3463812..3465185 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
R5031_RS16130 (R5031_16130)	-	3465428..3465832 (-)	405	WP_003084560.1	NINE protein	-
R5031_RS16135 (R5031_16135)	czcD	3465986..3466885 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
R5031_RS16140 (R5031_16140)	pilU	3466893..3468041 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5031_RS16145 (R5031_16145)	pilT	3468219..3469253 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5031_RS16150 (R5031_16150)	-	3469468..3470160 (+)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5031_RS16155 (R5031_16155)	proC	3470172..3470993 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5031_RS16160 (R5031_16160)	-	3471004..3471597 (+)	594	WP_003084544.1	YggT family protein	-
R5031_RS16165 (R5031_16165)	-	3471594..3471890 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898686 R5031_RS16140 WP_003100959.1 3466893..3468041(-) (pilU) [Pseudomonas aeruginosa strain HPA0271]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898686 R5031_RS16140 WP_003100959.1 3466893..3468041(-) (pilU) [Pseudomonas aeruginosa strain HPA0271]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382