Detailed information NTG0089649

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LDN16_RS04910
• Genome accession: NZ_AP024961
• Coordinates: 1077219..1078283 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain 80-045
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1072219..1083283

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LDN16_RS04900 (LEG80045_09460)	-	1073259..1075643 (-)	2385	WP_061637671.1	penicillin-binding protein 1A	-
LDN16_RS04905 (LEG80045_09470)	ravI	1075795..1076802 (-)	1008	WP_061637670.1	Dot/Icm T4SS effector RavI	-
LDN16_RS04910 (LEG80045_09480)	pilM	1077219..1078283 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
LDN16_RS04915 (LEG80045_09490)	pilN	1078287..1078835 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
LDN16_RS04920 (LEG80045_09500)	pilO	1078849..1079448 (+)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
LDN16_RS04925 (LEG80045_09510)	pilP	1079445..1080029 (+)	585	WP_027266404.1	pilus assembly protein PilP	Machinery gene
LDN16_RS04930 (LEG80045_09520)	pilQ	1080033..1082129 (+)	2097	WP_011215098.1	type IV pilus secretin PilQ	Machinery gene
LDN16_RS04935 (LEG80045_09530)	aroK	1082575..1083102 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=89649 LDN16_RS04910 WP_011215094.1 1077219..1078283(+) (pilM) [Legionella pneumophila strain 80-045]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=89649 LDN16_RS04910 WP_011215094.1 1077219..1078283(+) (pilM) [Legionella pneumophila strain 80-045]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407