Detailed information NTG0896147

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: R4701_RS00660
• Genome accession: NZ_CP137114
• Coordinates: 140608..142848 (-)
• Length: 746 a.a.
• NCBI ID: WP_000942406.1
• Uniprot ID: A0A4J2I0U7
• Organism: Streptococcus pneumoniae strain 15P3054
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 135608..147848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4701_RS00625	-	136518..136898 (-)	381	WP_000157154.1	VOC family protein	-
R4701_RS00630	rplT	136956..137315 (-)	360	WP_000124836.1	50S ribosomal protein L20	-
R4701_RS00635	rpmI	137367..137567 (-)	201	WP_001125943.1	50S ribosomal protein L35	-
R4701_RS00640	infC	137600..138130 (-)	531	WP_000848180.1	translation initiation factor IF-3	-
R4701_RS00645	-	138437..139618 (-)	1182	WP_000655934.1	hypothetical protein	-
R4701_RS00650	-	139622..140209 (-)	588	WP_000939880.1	ATP-binding cassette domain-containing protein	-
R4701_RS00655	-	140242..140459 (-)	218	Protein_130	hypothetical protein	-
R4701_RS00660	comEC/celB	140608..142848 (-)	2241	WP_000942406.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
R4701_RS00665	comEA/celA/cilE	142832..143482 (-)	651	WP_000387330.1	ComEA family DNA-binding protein	Machinery gene
R4701_RS00670	-	143550..144119 (-)	570	WP_000443899.1	GNAT family N-acetyltransferase	-
R4701_RS00675	ald	144296..145407 (+)	1112	Protein_134	alanine dehydrogenase	-
R4701_RS00680	-	145708..146034 (-)	327	Protein_135	TfoX/Sxy family protein	-
R4701_RS00685	-	146037..146537 (-)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
R4701_RS00690	-	146730..147698 (-)	969	WP_000658183.1	PhoH family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84545.06 Da
• Isoelectric Point: 9.5148

>NTDB_id=896147 R4701_RS00660 WP_000942406.1 140608..142848(-) (comEC/celB) [Streptococcus pneumoniae strain 15P3054]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTVKVNGDSLSFRGKADGRIFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFFTAGGVLSCAYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLTFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLISLALVYDLRKNIKKLTVLCLLITGLFLLTK
HPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
ENVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=896147 R4701_RS00660 WP_000942406.1 140608..142848(-) (comEC/celB) [Streptococcus pneumoniae strain 15P3054]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCCTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTCTGCCTGACACTGTTAAGGTCAATGGTGATAGTCTGTCCTTTCGCGGCAA
GGCTGATGGACGCATTTTTCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACCGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAAAAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGCGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGCATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTTACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTGCTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTATTTGACTTGACCTTCTTACCGCTCTTGTCTATT
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTGGAGGGCATTATTCGCTTGGT
GTCACAGGTGACAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGTTTTTAATCCTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGAAAAAACATTAAAAAGCTAACGGTATTGTGCTTATTGATTACAGGGCTCTTTCTCCTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGAATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTACCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-693)

• Domain of unknown function (DUF4131) (DUF4131) (31-177)

• Competence protein (Competence) (216-458)

3D structure

• AlphaFold DB: A0A4J2I0U7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae Rx1	98.928	100	0.989
comEC/celB	Streptococcus pneumoniae D39	98.928	100	0.989
comEC/celB	Streptococcus pneumoniae R6	98.928	100	0.989
comEC/celB	Streptococcus pneumoniae TIGR4	97.319	100	0.973
comEC/celB	Streptococcus mitis NCTC 12261	92.349	99.866	0.922
comEC/celB	Streptococcus mitis SK321	92.225	100	0.922
comEC	Lactococcus lactis subsp. cremoris KW2	43.725	99.33	0.434