Detailed information NTG0894483

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: AAVB98_RS00750
• Genome accession: NZ_CP154895
• Coordinates: 146145..148556 (-)
• Length: 803 a.a.
• NCBI ID: WP_061573883.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain Fad 77
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 141145..153556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVB98_RS00725 (AAVB98_00725)	-	141489..142250 (-)	762	WP_013352219.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
AAVB98_RS00730 (AAVB98_00730)	rlmN	142256..143347 (-)	1092	WP_013352218.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
AAVB98_RS00735 (AAVB98_00735)	rsmB	143351..144694 (-)	1344	WP_061573886.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
AAVB98_RS00740 (AAVB98_00740)	fmt	144681..145634 (-)	954	WP_061573885.1	methionyl-tRNA formyltransferase	-
AAVB98_RS00745 (AAVB98_00745)	def	145639..146121 (-)	483	WP_061573884.1	peptide deformylase	-
AAVB98_RS00750 (AAVB98_00750)	priA	146145..148556 (-)	2412	WP_061573883.1	primosomal protein N'	Machinery gene
AAVB98_RS00755 (AAVB98_00755)	coaBC	148553..149773 (-)	1221	WP_013352213.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
AAVB98_RS00760 (AAVB98_00760)	rpoZ	149864..150067 (-)	204	WP_003154346.1	DNA-directed RNA polymerase subunit omega	-
AAVB98_RS00765 (AAVB98_00765)	gmk	150071..150685 (-)	615	WP_013352212.1	guanylate kinase	-
AAVB98_RS00770 (AAVB98_00770)	remA	150693..150962 (-)	270	WP_003154355.1	extracellular matrix/biofilm regulator RemA	-
AAVB98_RS00775 (AAVB98_00775)	-	151039..151914 (-)	876	WP_013352211.1	YicC/YloC family endoribonuclease	-

Sequence

Protein

• Length: 803 a.a.
• Molecular weight: 90944.00 Da
• Isoelectric Point: 8.8944

>NTDB_id=894483 AAVB98_RS00750 WP_061573883.1 146145..148556(-) (priA) [Bacillus amyloliquefaciens strain Fad 77]
MNVAEVIVDVSSKNIDRPFDYRIPDHLRGMIEVGMRVIVPFGPRKLQGFVTGLKDSSELNGGQMKEVEKLLDLTPVLTEE
LMQLSSWLSDKTLSFKITALQAMLPAALKAKYEKELKVVDEEALTPDIRQLFRNQKSLLYSDITDERILSALQKFVRKGS
IDVTYKVAQKTNKKMIRMIEAGADKEELARHADSLTKQASKQLAVLTYFIDGGAKISAAELCQKTDSSSATLKTLLKKGL
LTDSFEEVYRDPYQDRMFKKTEPLSLTEEQQAAFEPIKKAVADHVHHVFLLHGVTGSGKTEIYLQSIEKVLAKGKEAIVL
VPEISLTPQMVNRFKGRFGSQVAVMHSGLSTGEKYDEWRKIQRKEVKLVVGARSAIFAPFENLGMIIIDEEHESSYKQEE
MPRYHAKDVAIRRAEHHSCPVVLGSATPTLESFARAKKGVYELLPLKHRVNRQLMPDVSLVDMREELRNGNRSMFSKELM
EQLEEKLAKKEQAVLFLNKRGYSSFVMCRDCGYVPQCPHCDISMTYHRYGQRLKCHYCGHEEPVPGTCPECGSEHIRFFG
TGTQRVEEELTKVLPQARVIRMDVDTTSRKGAHEKLLSAFGEGKADILLGTQMIAKGLDFPNVTLVGVLSADTTLHIPDF
RAAEKTFQLLTQVSGRAGRHEKPGTVVIQTYTPSHYSIQLTKTHDYETFFQQEMAHRRAQSYPPYYYVALVTVSHEEAAK
AAVTAEKITNYLRAGCGPDTKILGPAASPIARIKDRYRYQCVIKYRQENELQVQLKKILEHYKRDIAQKHVMISIDMNPY
MLM

Nucleotide

• Length: 2412 bp

>NTDB_id=894483 AAVB98_RS00750 WP_061573883.1 146145..148556(-) (priA) [Bacillus amyloliquefaciens strain Fad 77]
ATGAATGTGGCAGAAGTCATTGTCGATGTCAGCTCCAAAAACATTGACAGGCCCTTTGATTACAGAATTCCCGATCATTT
AAGAGGGATGATCGAAGTCGGCATGCGGGTGATTGTCCCGTTCGGCCCGCGCAAGCTTCAAGGGTTCGTGACCGGTTTGA
AAGATTCGTCTGAATTAAACGGCGGGCAAATGAAGGAAGTCGAGAAGCTTCTTGATTTGACACCCGTTTTGACGGAAGAG
CTGATGCAGCTGTCTTCATGGCTTTCTGACAAAACGCTGTCGTTTAAAATCACGGCGCTTCAGGCAATGCTGCCGGCCGC
GCTGAAGGCGAAGTACGAGAAAGAGCTGAAAGTCGTTGACGAAGAAGCGCTGACACCGGACATCAGGCAGCTTTTCAGAA
ATCAGAAGAGCCTTCTGTACTCAGATATTACCGATGAACGCATTCTTTCGGCACTGCAAAAATTCGTGCGCAAAGGAAGC
ATTGATGTCACCTATAAAGTCGCCCAGAAAACGAATAAAAAAATGATCCGCATGATTGAGGCGGGGGCAGACAAAGAAGA
ACTCGCCCGGCACGCAGATTCATTGACAAAACAGGCTTCTAAACAGCTTGCCGTGCTTACTTATTTTATTGACGGTGGAG
CAAAGATTTCCGCGGCTGAGCTTTGCCAAAAGACCGATTCCAGTTCAGCGACGCTGAAAACGCTTCTTAAAAAGGGGCTT
CTTACGGATAGCTTTGAGGAGGTTTACCGCGATCCTTATCAGGACAGAATGTTCAAAAAAACAGAACCTCTCTCTCTTAC
TGAAGAGCAACAGGCCGCTTTTGAGCCGATTAAGAAGGCCGTAGCCGATCATGTTCATCACGTCTTTCTGCTTCACGGTG
TGACAGGGAGCGGCAAAACGGAAATTTACCTGCAATCTATTGAAAAGGTGCTGGCTAAAGGAAAAGAAGCCATCGTGCTC
GTTCCGGAAATCTCATTGACGCCGCAAATGGTCAATCGTTTTAAAGGCCGCTTCGGTTCACAGGTTGCGGTCATGCACAG
CGGATTGTCCACAGGTGAAAAATACGATGAATGGCGGAAAATTCAGCGTAAGGAAGTAAAGCTTGTCGTCGGAGCCAGGT
CGGCCATCTTTGCCCCATTTGAAAATCTCGGCATGATCATTATTGATGAAGAGCACGAATCCTCCTATAAGCAGGAGGAA
ATGCCGCGGTACCATGCAAAAGACGTAGCCATACGCAGGGCCGAGCATCACAGCTGTCCGGTCGTCCTCGGGAGCGCCAC
GCCGACATTGGAGTCATTCGCACGGGCGAAAAAAGGCGTTTATGAGCTGCTCCCGCTGAAACACCGCGTAAATCGCCAGC
TCATGCCGGATGTATCTCTCGTTGATATGAGAGAAGAACTGCGAAACGGAAACAGATCGATGTTTTCAAAAGAGCTGATG
GAACAGCTGGAGGAGAAGCTTGCGAAAAAAGAGCAGGCCGTTCTCTTTCTGAATAAACGGGGATATTCTTCGTTTGTGAT
GTGCCGGGACTGCGGTTATGTGCCCCAATGTCCGCATTGCGACATTTCAATGACCTATCACCGCTACGGACAAAGGCTCA
AATGCCATTACTGCGGTCATGAGGAGCCTGTTCCGGGAACGTGCCCGGAATGCGGCAGTGAGCATATCCGCTTTTTCGGC
ACGGGAACGCAGCGTGTAGAGGAGGAGCTTACCAAAGTGCTGCCTCAGGCGCGCGTGATCAGGATGGATGTGGACACGAC
CTCAAGAAAAGGCGCTCATGAAAAGCTATTGTCAGCTTTCGGTGAAGGAAAAGCGGATATCCTGCTCGGTACGCAAATGA
TCGCAAAAGGGCTTGATTTTCCGAACGTGACGCTCGTCGGAGTATTAAGCGCAGATACGACGCTTCACATCCCTGATTTC
AGGGCGGCGGAGAAGACATTTCAGCTGTTGACGCAGGTAAGCGGACGGGCCGGGCGGCATGAAAAGCCGGGAACGGTAGT
CATACAAACGTACACACCGTCTCATTACAGCATTCAGCTGACGAAAACGCATGACTATGAAACTTTCTTTCAGCAGGAGA
TGGCGCACAGGCGGGCCCAGTCATATCCGCCTTACTATTATGTGGCGCTGGTGACCGTTTCTCATGAAGAAGCGGCAAAA
GCCGCGGTCACCGCTGAAAAGATTACAAACTATTTAAGAGCGGGATGCGGACCGGACACGAAAATTCTCGGGCCGGCGGC
TTCCCCGATTGCCAGGATCAAAGATAGATATCGCTATCAATGCGTGATAAAATACAGACAGGAAAACGAGTTGCAGGTTC
AGTTGAAAAAAATTCTGGAGCATTATAAACGCGACATTGCGCAAAAGCATGTAATGATTTCAATTGATATGAATCCGTAC
ATGCTGATGTAA

Domains

Predicted by InterProScan.

• Helicase conserved C-terminal domain (Helicase_C) (562-660)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (517-543)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-105)

• Primosomal protein N C-terminal domain (PriA_C) (704-799)

• Type III restriction enzyme, res subunit (ResIII) (265-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	83.727	100	0.839