Detailed information NTG0893909

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R3J35_RS03520
• Genome accession: NZ_CP136972
• Coordinates: 775533..776663 (-)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8173
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 770533..781663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R3J35_RS03500 (R3J35_03495)	-	770567..771325 (+)	759	WP_027700753.1	LytTR family DNA-binding domain-containing protein	-
R3J35_RS03505 (R3J35_03500)	hemC	771333..772250 (+)	918	WP_004084955.1	hydroxymethylbilane synthase	-
R3J35_RS03510 (R3J35_03505)	-	772304..773113 (-)	810	WP_021358437.1	YdiY family protein	-
R3J35_RS03515 (R3J35_03510)	-	773896..774864 (+)	969	WP_004084952.1	YafY family protein	-
R3J35_RS03520 (R3J35_03515)	pilU	775533..776663 (-)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R3J35_RS03525 (R3J35_03520)	pilT	776818..777852 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
R3J35_RS03530 (R3J35_03525)	-	778474..778686 (+)	213	WP_021358439.1	hypothetical protein	-
R3J35_RS03535 (R3J35_03530)	-	779071..779898 (+)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
R3J35_RS03540 (R3J35_03535)	-	780528..781352 (-)	825	WP_004084943.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=893909 R3J35_RS03520 WP_004084950.1 775533..776663(-) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=893909 R3J35_RS03520 WP_004084950.1 775533..776663(-) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402