Detailed information NTG0889474

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWT85_RS13735
• Genome accession: NZ_CP136179
• Coordinates: 2898476..2899594 (+)
• Length: 372 a.a.
• NCBI ID: WP_317856483.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain ABO21-A022
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2893476..2904594

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWT85_RS13725 (RWT85_13725)	-	2896592..2897284 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
RWT85_RS13730 (RWT85_13730)	pilT	2897411..2898448 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWT85_RS13735 (RWT85_13735)	pilU	2898476..2899594 (+)	1119	WP_317856483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWT85_RS13740 (RWT85_13740)	fur	2899664..2900101 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWT85_RS13745 (RWT85_13745)	-	2900213..2900611 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWT85_RS13750 (RWT85_13750)	-	2900666..2900989 (-)	324	WP_000442587.1	RnfH family protein	-
RWT85_RS13755 (RWT85_13755)	-	2900989..2902056 (-)	1068	WP_002019572.1	hypothetical protein	-
RWT85_RS13760 (RWT85_13760)	-	2902209..2902664 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RWT85_RS13765 (RWT85_13765)	-	2902917..2903765 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41947.15 Da
• Isoelectric Point: 6.6945

>NTDB_id=889474 RWT85_RS13735 WP_317856483.1 2898476..2899594(+) (pilU) [Acinetobacter baumannii strain ABO21-A022]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFEQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889474 RWT85_RS13735 WP_317856483.1 2898476..2899594(+) (pilU) [Acinetobacter baumannii strain ABO21-A022]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGAGCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392