Detailed information NTG0887686

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RS899_RS29145
• Genome accession: NZ_CP135919
• Coordinates: 6344438..6345583 (+)
• Length: 381 a.a.
• NCBI ID: WP_024766757.1
• Uniprot ID: -
• Organism: Pseudomonas sp. C9-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6339438..6350583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RS899_RS29120	-	6340740..6341030 (-)	291	WP_315808664.1	DUF167 domain-containing protein	-
RS899_RS29125	-	6341081..6341674 (-)	594	WP_169934999.1	YggT family protein	-
RS899_RS29130	proC	6341688..6342518 (-)	831	WP_315808665.1	pyrroline-5-carboxylate reductase	-
RS899_RS29135	-	6342536..6343225 (-)	690	WP_315808666.1	YggS family pyridoxal phosphate-dependent enzyme	-
RS899_RS29140	pilT	6343299..6344333 (+)	1035	WP_024766758.1	type IV pilus twitching motility protein PilT	Machinery gene
RS899_RS29145	pilU	6344438..6345583 (+)	1146	WP_024766757.1	type IV pilus ATPase PilU	Machinery gene
RS899_RS29150	-	6345653..6346057 (+)	405	WP_054907821.1	NINE protein	-
RS899_RS29155	-	6346281..6347654 (+)	1374	WP_315808667.1	cystathionine beta-synthase	-
RS899_RS29160	-	6347651..6348820 (+)	1170	WP_315808668.1	PLP-dependent aspartate aminotransferase family protein	-
RS899_RS29165	-	6349142..6350413 (-)	1272	WP_054907824.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42584.91 Da
• Isoelectric Point: 6.7255

>NTDB_id=887686 RS899_RS29145 WP_024766757.1 6344438..6345583(+) (pilU) [Pseudomonas sp. C9-3]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAYADSANDLRLMIKLGSETDADHLTTMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=887686 RS899_RS29145 WP_024766757.1 6344438..6345583(+) (pilU) [Pseudomonas sp. C9-3]
ATGGAATTCGAGAAGCTGTTGCGCCTGATGGTGGAAAAGGGTGCCTCCGACCTGTTCATCACAGCGGGCGTACCACCGTC
GATGAAGGTCAACGGCAAGGTGTTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAAACCGTGCTGTCGG
TGATGAACGAGCAGCAGCGTCGTGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCGTCGGTCGT
TTCCGCGTCAGCGCTTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCATCGA
TGACCTGAAGCTGCCGGAAATCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTATCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAT
CCCATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAAGTGGGCCTGGATACCGATACCTTCGAAGT
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCCGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAACTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACTTACGAAGATGCGCTGGCCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTGGGTTCGGAAACCGACGCCGACCACCTGACCACCATGACCCAGGGCCTGACCCTGGAGATGAGCGACG
ACGATCCCAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.451	100	0.885
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	60.286	91.864	0.554
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383