Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RQR14_RS05050 Genome accession   NZ_CP135196
Coordinates   1043522..1044907 (+) Length   461 a.a.
NCBI ID   WP_014668226.1    Uniprot ID   A0A849CCS0
Organism   Pasteurella multocida strain 80176     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038522..1049907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQR14_RS05020 - 1038712..1039563 (-) 852 WP_014668231.1 thymidylate synthase -
  RQR14_RS05025 lgt 1039569..1040372 (-) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  RQR14_RS05030 - 1040382..1041176 (-) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  RQR14_RS05035 rppH 1041308..1041775 (-) 468 Protein_981 RNA pyrophosphohydrolase -
  RQR14_RS05040 ampD 1042361..1042927 (-) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RQR14_RS05045 pilA 1043088..1043522 (+) 435 WP_014668227.1 prepilin peptidase-dependent pilin Machinery gene
  RQR14_RS05050 pilB 1043522..1044907 (+) 1386 WP_014668226.1 GspE/PulE family protein Machinery gene
  RQR14_RS05055 - 1044907..1046118 (+) 1212 WP_014668225.1 type II secretion system F family protein -
  RQR14_RS05060 - 1046118..1046840 (+) 723 WP_016534448.1 A24 family peptidase -
  RQR14_RS05065 coaE 1046855..1047475 (+) 621 WP_005751078.1 dephospho-CoA kinase -
  RQR14_RS05070 yacG 1047486..1047689 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RQR14_RS05075 - 1047689..1047958 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RQR14_RS05080 - 1048407..1049780 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52108.79 Da        Isoelectric Point: 6.3686

>NTDB_id=886163 RQR14_RS05050 WP_014668226.1 1043522..1044907(+) (pilB) [Pasteurella multocida strain 80176]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIELVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=886163 RQR14_RS05050 WP_014668226.1 1043522..1044907(+) (pilB) [Pasteurella multocida strain 80176]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCTTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTTGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACATTGC
CGACACATTTAGGTGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGTCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A849CCS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

39.484

100

0.432
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.412
  pilB Acinetobacter baylyi ADP1

37.675

100

0.408
  pilB Vibrio cholerae strain A1552

38.477

100

0.406
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395