Detailed information NTG0088542

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: OCUAc18_RS01670
• Genome accession: NZ_AP024802
• Coordinates: 345087..346799 (-)
• Length: 570 a.a.
• NCBI ID: WP_001274979.1
• Uniprot ID: A0A3R9T4L6
• Organism: Acinetobacter baumannii strain OCU Ac18
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	347329..347931	345087..346799	flank	530

Gene organization within MGE regions

Location: 345087..347931

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCUAc18_RS01670 (OCUAc18_03170)	pilB	345087..346799 (-)	1713	WP_001274979.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OCUAc18_RS01675	-	347062..347931 (+)	870	WP_016653396.1	IS982-like element ISAcsp2 family transposase	-

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 63324.70 Da
• Isoelectric Point: 5.3317

>NTDB_id=88542 OCUAc18_RS01670 WP_001274979.1 345087..346799(-) (pilB) [Acinetobacter baumannii strain OCU Ac18]
MSALHTSPKFTGFFRRLVEEKHVSAAAMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVNQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide

• Length: 1713 bp

>NTDB_id=88542 OCUAc18_RS01670 WP_001274979.1 345087..346799(-) (pilB) [Acinetobacter baumannii strain OCU Ac18]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGGC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACCTCTCAGATT
CCTAAAGATCTGGTCAATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGCCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCACAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCTATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGCGTGTTACGCCAAATTGCTAATCCCCCTCTACAGCTTGCAAACCGCTTAGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGTGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AAGCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAACCTTGAAGGAATTAATCAAGTTAACGTAAACCCCA
AAGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-151)

• Type II/IV secretion system protein (T2SSE) (192-459)

3D structure

• AlphaFold DB: A0A3R9T4L6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	99.474	100	0.995
pilB	Acinetobacter baylyi ADP1	79.649	100	0.796
pilB	Legionella pneumophila strain ERS1305867	50.97	99.474	0.507
pilF	Neisseria gonorrhoeae MS11	50.356	98.596	0.496
pilB	Vibrio parahaemolyticus RIMD 2210633	51.698	92.982	0.481
pilB	Vibrio campbellii strain DS40M4	50.867	91.053	0.463
pilB	Vibrio cholerae strain A1552	50.29	90.702	0.456
pilB/pilB1	Synechocystis sp. PCC 6803	35.179	100	0.379