Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RN346_RS08400 Genome accession   NZ_CP134865
Coordinates   1832803..1834548 (+) Length   581 a.a.
NCBI ID   WP_311962674.1    Uniprot ID   -
Organism   Halomonas sp. PAMB 3232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1827803..1839548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN346_RS08355 (RN346_08355) - 1828215..1828940 (+) 726 WP_311962670.1 sulfite exporter TauE/SafE family protein -
  RN346_RS08360 (RN346_08360) - 1829038..1829241 (+) 204 WP_263587244.1 hypothetical protein -
  RN346_RS08365 (RN346_08365) - 1829335..1829514 (+) 180 WP_263587243.1 hypothetical protein -
  RN346_RS08375 (RN346_08375) - 1829750..1830553 (-) 804 WP_311962671.1 inositol monophosphatase family protein -
  RN346_RS08380 (RN346_08380) - 1830619..1831071 (-) 453 WP_311962672.1 anti-virulence regulator CigR family protein -
  RN346_RS08385 (RN346_08385) - 1831386..1831520 (-) 135 WP_311960801.1 hypothetical protein -
  RN346_RS08390 (RN346_08390) - 1831594..1831875 (-) 282 Protein_1649 anti-virulence regulator CigR family protein -
  RN346_RS08395 (RN346_08395) - 1832150..1832581 (-) 432 WP_311962673.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RN346_RS08400 (RN346_08400) pilB 1832803..1834548 (+) 1746 WP_311962674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RN346_RS08405 (RN346_08405) - 1834557..1835783 (+) 1227 WP_263587239.1 type II secretion system F family protein -
  RN346_RS08410 (RN346_08410) - 1835837..1836682 (+) 846 WP_311962675.1 A24 family peptidase -
  RN346_RS08415 (RN346_08415) coaE 1836711..1837337 (+) 627 WP_311962676.1 dephospho-CoA kinase -
  RN346_RS08420 (RN346_08420) yacG 1837395..1837625 (+) 231 WP_263587236.1 DNA gyrase inhibitor YacG -
  RN346_RS08425 (RN346_08425) - 1837671..1838543 (+) 873 WP_311960806.1 DNA replication terminus site-binding protein -
  RN346_RS08430 (RN346_08430) - 1838515..1838865 (-) 351 WP_311960807.1 hypothetical protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63594.92 Da        Isoelectric Point: 5.3284

>NTDB_id=884174 RN346_RS08400 WP_311962674.1 1832803..1834548(+) (pilB) [Halomonas sp. PAMB 3232]
MSSSAFDQPLASRALSGIAKRLVAHEVMTQGQAEVADTTSAELEVSFLEHVVQSGLVGSVDAALAAAWEYGLPVVDLEAI
KISALPPASDYPVKILEKLNVLPLARHGHRLTVAVPYPATLTRLDELQFATSLSVEGVLAPVEQIRNNLAQYVSQNERSL
LDELEGVDDAVGELNVVQQEEDEEVTLESSVTNKDDAPVVKFVNKVLLDAIGRGASDIHFEPFESQYRVRFRIDGMLIEV
AKPPFSMRDRIAARLKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVYGEKLVLRILDPSSVRLGIEQLGFSP
TQQREYEYALKRPQGMILVTGPTGSGKTVTLYTGINMLNEIERNICTAEDPVEIKVSGINQVNVLPKIGLNFASALRAFL
RQDPDVVMVGEIRDLETAEIAVKAAQTGHMVLSTVHTNSAAQTLTRLANMGVASYNIASSVTLIIAQRLARRLCPSCKAP
ADIPQEALLKAGFSKEQLTNVTIYKAVGCKQCTQGYKGRVGIYEVLPISDAMRNLIMRDGNALEIDELARKEGHPSLYQS
CLARVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=884174 RN346_RS08400 WP_311962674.1 1832803..1834548(+) (pilB) [Halomonas sp. PAMB 3232]
ATGTCCTCATCCGCCTTCGATCAGCCCTTGGCTTCACGCGCGCTTAGCGGTATCGCGAAACGGCTCGTCGCTCATGAGGT
GATGACCCAGGGGCAGGCCGAAGTCGCCGATACGACGTCTGCCGAGCTCGAAGTCTCCTTTCTGGAACACGTGGTACAAA
GCGGGCTGGTGGGCTCGGTCGATGCAGCGCTCGCGGCGGCCTGGGAGTACGGTTTGCCGGTGGTAGATCTGGAGGCGATC
AAGATCAGCGCGCTTCCCCCGGCCAGCGACTATCCGGTCAAGATTCTCGAAAAGCTGAACGTTTTGCCGCTGGCACGCCA
CGGCCACCGGCTGACCGTAGCAGTCCCCTACCCGGCCACGCTCACACGGCTCGACGAGCTTCAGTTTGCGACCAGCCTGA
GTGTCGAGGGCGTGCTGGCGCCGGTCGAGCAGATACGCAATAACCTGGCGCAGTACGTCTCGCAAAACGAGCGCAGCCTG
CTGGATGAGTTGGAAGGCGTGGACGACGCCGTGGGCGAGCTCAATGTCGTGCAGCAGGAAGAGGATGAAGAGGTTACGCT
CGAGTCCTCGGTGACGAACAAGGATGACGCACCGGTCGTGAAGTTCGTCAATAAGGTGCTGCTGGACGCCATCGGCCGCG
GGGCGTCGGATATTCACTTCGAGCCGTTCGAATCCCAGTACCGGGTTCGCTTTCGTATCGACGGCATGCTCATCGAGGTC
GCTAAACCGCCCTTTTCGATGCGCGACCGAATCGCCGCACGCCTGAAGATCATGTCGCGACTCGATATCTCCGAGCGTCG
CCTGCCCCAGGACGGCGCGATCAAGCTTCAGCTCTCGCGCACCCGCTCGATCGACTTTCGCGTCAACTCACTGCCCACCG
TGTATGGCGAAAAGCTGGTGCTGCGGATACTCGACCCCTCCTCGGTGCGACTCGGCATCGAGCAGCTGGGCTTTAGCCCC
ACACAGCAGCGCGAGTACGAATACGCGCTCAAGCGCCCTCAGGGCATGATTCTGGTCACCGGGCCAACCGGCAGCGGCAA
GACCGTGACACTTTATACCGGCATCAACATGCTCAATGAGATCGAGCGCAATATTTGTACCGCTGAGGATCCGGTCGAGA
TCAAGGTGTCCGGCATCAACCAGGTCAACGTGCTGCCCAAAATCGGACTCAACTTTGCCAGTGCCCTACGGGCGTTTTTG
CGCCAGGATCCGGACGTGGTCATGGTCGGCGAGATTCGCGATCTCGAAACCGCCGAGATCGCCGTCAAGGCGGCGCAAAC
CGGCCACATGGTGCTCTCCACGGTTCACACCAATTCGGCGGCGCAAACCCTGACGCGCCTGGCCAACATGGGGGTGGCCT
CTTATAACATCGCAAGTTCGGTCACGCTGATTATTGCTCAGCGCCTTGCGCGCCGGCTTTGCCCGAGCTGCAAAGCGCCG
GCGGACATTCCTCAGGAAGCGCTTTTAAAAGCCGGCTTTAGCAAGGAGCAGCTGACCAACGTGACGATCTATAAAGCGGT
TGGCTGTAAACAGTGCACTCAGGGGTACAAGGGCCGGGTCGGTATTTATGAAGTGCTGCCGATCAGCGATGCCATGCGCA
ACCTGATCATGCGTGACGGCAACGCACTCGAAATCGACGAGCTGGCGCGCAAGGAGGGCCACCCGAGCCTTTACCAAAGC
TGTCTTGCGCGCGTACTCGATGGCAGCACGAGCCTTGAAGAAGTCAACCGTATCAGCAAGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-156)

Type II/IV secretion system protein (T2SSE)

(205-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.499

97.59

0.503
  pilB Acinetobacter baumannii D1279779

49.65

98.451

0.489
  pilB Acinetobacter baylyi ADP1

47.619

100

0.482
  pilB Vibrio cholerae strain A1552

51.562

88.124

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

48.699

92.599

0.451
  pilB Vibrio campbellii strain DS40M4

48.333

92.943

0.449
  pilF Neisseria gonorrhoeae MS11

45.39

97.074

0.441
  pilB/pilB1 Synechocystis sp. PCC 6803

38.949

95.009

0.37