Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RH061_RS16075 Genome accession   NZ_CP134494
Coordinates   3185445..3186521 (-) Length   358 a.a.
NCBI ID   WP_311071652.1    Uniprot ID   -
Organism   Mesobacillus jeotgali strain PS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3180445..3191521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RH061_RS16030 (RH061_16030) - 3180925..3181713 (+) 789 WP_311071639.1 YqhG family protein -
  RH061_RS16035 (RH061_16035) - 3181766..3181948 (-) 183 WP_311071641.1 YqzE family protein -
  RH061_RS16040 (RH061_16040) - 3181990..3182490 (-) 501 WP_311071642.1 shikimate kinase -
  RH061_RS16045 (RH061_16045) comGG 3182507..3182818 (-) 312 WP_311071644.1 competence type IV pilus minor pilin ComGG -
  RH061_RS16050 (RH061_16050) comGF 3182878..3183423 (-) 546 WP_311071646.1 competence type IV pilus minor pilin ComGF -
  RH061_RS16055 (RH061_16055) - 3183305..3183640 (-) 336 WP_311071647.1 hypothetical protein -
  RH061_RS16060 (RH061_16060) comGD 3183624..3184040 (-) 417 WP_311071648.1 competence type IV pilus minor pilin ComGD -
  RH061_RS16065 (RH061_16065) comGC 3184061..3184387 (-) 327 WP_311076428.1 competence type IV pilus major pilin ComGC -
  RH061_RS16070 (RH061_16070) comGB 3184427..3185458 (-) 1032 WP_311071650.1 competence type IV pilus assembly protein ComGB -
  RH061_RS16075 (RH061_16075) comGA 3185445..3186521 (-) 1077 WP_311071652.1 competence type IV pilus ATPase ComGA Machinery gene
  RH061_RS16085 (RH061_16085) - 3186764..3187117 (-) 354 WP_311071654.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RH061_RS16090 (RH061_16090) - 3187311..3188012 (+) 702 WP_311071656.1 helix-turn-helix domain-containing protein -
  RH061_RS16095 (RH061_16095) - 3188092..3188337 (+) 246 WP_023627332.1 DUF2626 domain-containing protein -
  RH061_RS16100 (RH061_16100) - 3188405..3189496 (-) 1092 WP_311071662.1 SAM-dependent methyltransferase -
  RH061_RS16105 (RH061_16105) - 3189665..3190300 (-) 636 WP_311071665.1 MBL fold metallo-hydrolase -
  RH061_RS16110 (RH061_16110) - 3190488..3190967 (+) 480 WP_311071667.1 hypothetical protein -
  RH061_RS16115 (RH061_16115) - 3191079..3191252 (+) 174 WP_311071668.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 40002.90 Da        Isoelectric Point: 7.4219

>NTDB_id=880441 RH061_RS16075 WP_311071652.1 3185445..3186521(-) (comGA) [Mesobacillus jeotgali strain PS1]
MIIVKSIEQLADNVLKDALRSGASDIHIIPREKDTLIKFRLGNQLLPRYTLEQADCERLISHFKFTASMDIGEKRRPQSG
AFTYQFEETKTGLRISTLPAYQSESMVIRLLPEQNQTPSFQISLFPSTSKKLISLLKHAHGLIIFTGPTGSGKTTTLYSM
LSETSELVNRNVITLEDPIEKPSDTVLQVQVNERAGISYSAGLKAILRHDPDIIMVGEIRDKETAETAIRASLTGHLVLT
TMHTRDARGAIYRLIEFGINWLEIEQTLIAVTAQRLVELTCPYCEGTCSPFCYSSGSGKRGNVFEILTGKDLSAVLKEAK
GESQSYHYKTLKDAIRKGIALGFIKESEYQRWVLNDGE

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=880441 RH061_RS16075 WP_311071652.1 3185445..3186521(-) (comGA) [Mesobacillus jeotgali strain PS1]
ATGATTATTGTCAAATCAATTGAACAGCTAGCGGACAATGTCCTGAAAGATGCTCTAAGAAGTGGCGCGTCGGATATTCA
TATCATCCCCCGTGAAAAAGACACACTTATAAAATTCAGGCTAGGCAATCAGCTTCTCCCCCGGTATACGCTTGAGCAGG
CAGATTGTGAACGGCTCATATCCCACTTCAAATTCACAGCTTCCATGGATATTGGTGAAAAAAGACGTCCTCAAAGCGGT
GCTTTTACTTATCAATTCGAAGAAACGAAGACTGGCTTAAGGATTTCCACGCTCCCAGCCTACCAGAGCGAAAGCATGGT
CATTCGACTCCTTCCCGAGCAAAATCAAACTCCTTCTTTCCAAATCTCATTATTCCCATCCACATCAAAAAAACTGATTT
CCCTTTTAAAGCATGCACATGGCTTGATCATTTTTACTGGACCTACTGGAAGTGGTAAGACGACTACCTTATATTCCATG
CTTTCTGAAACCTCGGAATTAGTCAATCGCAATGTAATCACCCTAGAAGATCCGATTGAAAAACCGAGTGACACAGTGCT
GCAGGTCCAGGTCAATGAACGGGCAGGCATTTCATATTCTGCCGGCTTGAAGGCTATTCTGCGGCATGATCCGGATATTA
TCATGGTTGGGGAAATTCGGGATAAAGAGACAGCCGAAACGGCTATTAGGGCTTCGCTGACAGGTCATTTAGTTCTGACC
ACTATGCATACACGTGATGCAAGGGGAGCGATATACAGGCTGATTGAATTCGGGATCAATTGGCTTGAGATTGAACAGAC
ATTGATTGCAGTAACGGCACAGAGGCTCGTTGAATTGACGTGTCCGTACTGTGAAGGAACTTGCTCGCCATTTTGCTATT
CATCAGGAAGCGGAAAAAGAGGAAATGTTTTCGAAATCCTGACGGGGAAGGATTTATCTGCCGTACTGAAGGAAGCAAAG
GGGGAGAGCCAAAGCTATCACTACAAGACGCTTAAAGATGCGATCCGGAAAGGAATCGCACTGGGGTTCATCAAGGAGTC
CGAATACCAGAGGTGGGTTCTCAATGATGGAGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.618

99.441

0.553
  pilB Glaesserella parasuis strain SC1401

39.542

97.486

0.385
  ctsE Campylobacter jejuni subsp. jejuni 81-176

37.143

97.765

0.363