Detailed information NTG0880147

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: RJW55_RS00815
• Genome accession: NZ_CP134473
• Coordinates: 134897..135331 (+)
• Length: 144 a.a.
• NCBI ID: WP_043026797.1
• Uniprot ID: A0A0Z8IB52
• Organism: Streptococcus suis strain TMW_SS028
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 129897..140331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RJW55_RS00780 (RJW55_00780)	-	130535..130894 (+)	360	WP_043026811.1	DUF1033 family protein	-
RJW55_RS00785 (RJW55_00785)	-	131039..131989 (-)	951	WP_043026809.1	S66 peptidase family protein	-
RJW55_RS00790 (RJW55_00790)	comYA	132075..133025 (+)	951	WP_043026808.1	competence type IV pilus ATPase ComGA	Machinery gene
RJW55_RS00795 (RJW55_00795)	comYB	132937..133974 (+)	1038	WP_043026806.1	competence type IV pilus assembly protein ComGB	Machinery gene
RJW55_RS00800 (RJW55_00800)	comYC	133976..134257 (+)	282	WP_043026803.1	competence type IV pilus major pilin ComGC	Machinery gene
RJW55_RS00805 (RJW55_00805)	comGD	134238..134645 (+)	408	WP_043026801.1	competence type IV pilus minor pilin ComGD	-
RJW55_RS00810 (RJW55_00810)	comYE	134617..134910 (+)	294	WP_043026799.1	competence type IV pilus minor pilin ComGE	Machinery gene
RJW55_RS00815 (RJW55_00815)	comGF/cglF	134897..135331 (+)	435	WP_043026797.1	competence type IV pilus minor pilin ComGF	Machinery gene
RJW55_RS00820 (RJW55_00820)	comGG	135309..135836 (+)	528	WP_043026795.1	competence type IV pilus minor pilin ComGG	-
RJW55_RS00825 (RJW55_00825)	comYH	135893..136846 (+)	954	WP_043026794.1	class I SAM-dependent methyltransferase	Machinery gene
RJW55_RS00830 (RJW55_00830)	-	136897..138084 (+)	1188	WP_043026792.1	acetate kinase	-
RJW55_RS00835 (RJW55_00835)	-	138399..138950 (+)	552	WP_043026790.1	folate family ECF transporter S component	-
RJW55_RS00840 (RJW55_00840)	-	139006..140262 (+)	1257	WP_043026788.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16462.85 Da
• Isoelectric Point: 8.8126

>NTDB_id=880147 RJW55_RS00815 WP_043026797.1 134897..135331(+) (comGF/cglF) [Streptococcus suis strain TMW_SS028]
MLKSKLPAFTLLECLVALIILSGSLLVFESLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDRGQGYQPMIYQVDSAAISQKNQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=880147 RJW55_RS00815 WP_043026797.1 134897..135331(+) (comGF/cglF) [Streptococcus suis strain TMW_SS028]
TTGTTAAAAAGTAAACTTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAAGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACTGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGCTATCAGCCCATGAT
TTATCAAGTAGACAGCGCAGCTATTTCCCAAAAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACGTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: A0A0Z8IB52

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae D39	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae R6	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae TIGR4	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae Rx1	53.901	97.917	0.528
comYF	Streptococcus mutans UA140	49.306	100	0.493
comGF/cglF	Streptococcus mitis NCTC 12261	52.985	93.056	0.493
comYF	Streptococcus mutans UA159	48.611	100	0.486
comGF/cglF	Streptococcus mitis SK321	51.493	93.056	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	43.972	97.917	0.431