Detailed information NTG0878577

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RG931_RS14615
• Genome accession: NZ_CP134066
• Coordinates: 3225586..3226755 (+)
• Length: 389 a.a.
• NCBI ID: WP_017682837.1
• Uniprot ID: A0A0K8M896
• Organism: Pseudomonas syringae pv. actinidiae strain QSY6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3220586..3231755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RG931_RS14600 (RG931_002921)	-	3222198..3222752 (+)	555	WP_017684828.1	hypothetical protein	-
RG931_RS14605 (RG931_002922)	ltrA	3223237..3224604 (-)	1368	WP_032685472.1	group II intron reverse transcriptase/maturase	-
RG931_RS14610 (RG931_002923)	-	3225156..3225431 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
RG931_RS14615 (RG931_002924)	pilU	3225586..3226755 (+)	1170	WP_017682837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RG931_RS14620 (RG931_002925)	-	3227695..3229272 (-)	1578	WP_017699732.1	ABC transporter ATP-binding protein	-
RG931_RS14625 (RG931_002926)	-	3229274..3230296 (-)	1023	WP_017682835.1	ABC transporter permease	-
RG931_RS14630 (RG931_002927)	-	3230296..3231360 (-)	1065	WP_003383303.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42669.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=878577 RG931_RS14615 WP_017682837.1 3225586..3226755(+) (pilU) [Pseudomonas syringae pv. actinidiae strain QSY6]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=878577 RG931_RS14615 WP_017682837.1 3225586..3226755(+) (pilU) [Pseudomonas syringae pv. actinidiae strain QSY6]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCAGCGAAACCCTCAAGCCCGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCCGGCATTGGTCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTTGATATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTATTGCTCGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGCAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGGAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATCAGCGAGGAAGAAGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTTCG
CCTCAAACTGCACAGCGAAAATGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A0K8M896

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365