Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   RIM63_RS08235 Genome accession   NZ_CP133952
Coordinates   1691854..1693749 (-) Length   631 a.a.
NCBI ID   WP_115251242.1    Uniprot ID   A0A380JRY0
Organism   Streptococcus equi subsp. zooepidemicus strain HT321     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1686854..1698749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIM63_RS08210 (RIM63_08210) - 1687598..1687960 (-) 363 WP_012516140.1 S1 RNA-binding domain-containing protein -
  RIM63_RS08215 (RIM63_08215) - 1687960..1689360 (-) 1401 WP_317583776.1 Cof-type HAD-IIB family hydrolase -
  RIM63_RS08220 (RIM63_08220) - 1689394..1690035 (-) 642 WP_021321066.1 response regulator -
  RIM63_RS08225 (RIM63_08225) - 1690028..1691032 (-) 1005 WP_012516143.1 sensor histidine kinase -
  RIM63_RS08230 (RIM63_08230) liaF 1691029..1691721 (-) 693 WP_012679994.1 cell wall-active antibiotics response protein LiaF -
  RIM63_RS08235 (RIM63_08235) pknB 1691854..1693749 (-) 1896 WP_115251242.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  RIM63_RS08240 (RIM63_08240) - 1693746..1694486 (-) 741 WP_111678385.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  RIM63_RS08245 (RIM63_08245) rsmB 1694524..1695846 (-) 1323 WP_317585294.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  RIM63_RS08250 (RIM63_08250) fmt 1695836..1696771 (-) 936 WP_037579761.1 methionyl-tRNA formyltransferase -

Sequence

Protein


Download         Length: 631 a.a.        Molecular weight: 68522.64 Da        Isoelectric Point: 6.5196

>NTDB_id=877778 RIM63_RS08235 WP_115251242.1 1691854..1693749(-) (pknB) [Streptococcus equi subsp. zooepidemicus strain HT321]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGSDLKKYIQDHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKSGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIDENPNVP
QALENVVIRATAKKLSDRYRSTFEMSRDLMTALSYNRSRERKVIFDDVESTKPLPKVTPAPITAPKVSAASQASGAADTR
PEQTSQMDTLQKPQKKKKNGRFLGTLLKILFSFFVVGVALFAYLVLTKPTTVKVPDVTGSSLNAAKQVLEEAGLKVGKVH
QIESDTVSEDQVVKTNPQAGSAKRKGSSVDLYLSIGNKGFEMENYKGQDYQDVMTSLTETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGEKFNPAGKSKITLSVAVGDTVIMPMVTEYSYADAVNTLTALGIDAARIKAYTPSSSSPTGFVQVNAPSA
KAIVSGQMPYYGTQISLSEKGDINLYLYPEETHSSSSSSSSDTSASNPSAGDTTDSSSHTELGQNDSAATP

Nucleotide


Download         Length: 1896 bp        

>NTDB_id=877778 RIM63_RS08235 WP_115251242.1 1691854..1693749(-) (pknB) [Streptococcus equi subsp. zooepidemicus strain HT321]
ATGATTCAGATTGGCAAGCTATTTGCTGGTCGTTATCGCATTCTAAAATCTATAGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAACGATTTGATTCTGGATAATGAGGACGTTGCCATTAAGGTCTTGCGGACCAATTATCAAACAGATCAGGTTG
CTGTTGCGCGTTTTCAAAGAGAAGCGCGAGCCATGGCTGAATTAAATCACCCCAATATTGTTGCCATTCGGGATATAGGC
GAGGAAGACGGACAGCAATTTTTAGTAATGGAATACGTTGATGGCTCTGACTTGAAAAAATACATTCAGGATCATGCGCC
ATTATCCAACAATGAGGTGGTGAGAATCATGGAGGAGGTTCTTTCTGCCATGACTCTGGCCCATCAAAAAGGAATTGTCC
ACAGGGATTTAAAGCCTCAAAACATTCTTTTAACCAAATCAGGTGTTGTTAAGGTTACAGATTTTGGGATTGCCGTTGCC
TTTGCAGAGACCAGCCTAACCCAGACAAATTCTATGCTAGGAAGTGTGCATTACCTCTCACCTGAGCAAGCGCGTGGTTC
TAAAGCGACGATTCAAAGTGATATTTACGCGATGGGAATCATGCTCTTTGAAATGCTAACAGGGCATATTCCTTATGATG
GCGATAGCGCTGTGACGATTGCTCTGCAGCATTTTCAAAAGCCGCTGCCCTCTATCATTGATGAAAATCCTAATGTGCCT
CAAGCTTTGGAAAATGTTGTTATTCGGGCCACTGCCAAAAAGCTAAGTGACCGTTATCGCTCGACCTTTGAGATGAGCAG
GGATTTGATGACAGCCCTTAGCTATAACCGCAGTCGTGAGCGTAAGGTTATTTTTGATGATGTCGAAAGCACAAAGCCAT
TACCAAAGGTCACACCAGCCCCTATCACAGCACCTAAGGTGTCGGCTGCCAGTCAAGCATCTGGAGCAGCTGATACTAGA
CCTGAGCAGACTAGCCAGATGGACACCCTGCAAAAGCCTCAAAAAAAGAAAAAAAATGGGCGCTTTTTAGGAACGCTTTT
AAAAATCTTGTTTTCCTTTTTCGTTGTCGGTGTGGCTCTTTTTGCTTATCTGGTCTTGACCAAGCCAACTACGGTTAAGG
TTCCTGATGTGACAGGCTCTAGTCTCAATGCTGCCAAGCAGGTTTTGGAAGAAGCAGGACTAAAGGTCGGTAAGGTCCAT
CAGATTGAAAGCGACACAGTCAGTGAGGATCAGGTGGTTAAGACAAATCCTCAAGCAGGCTCAGCCAAGCGAAAGGGCTC
CAGCGTTGACCTCTACCTTTCTATCGGAAATAAAGGCTTTGAGATGGAAAATTATAAGGGACAGGATTATCAGGATGTGA
TGACCAGTCTGACTGAAACCTACGGTGTACCAAAGTCAAAAATCAAGATTGAGCGTATCGTGACCAATGAATACCCTGAA
AACACCGTTATTAGCCAGTCACCAAGCGCGGGAGAAAAATTTAACCCAGCTGGTAAATCCAAAATTACGCTTAGTGTAGC
AGTGGGCGATACGGTCATCATGCCAATGGTAACTGAGTACAGCTATGCAGATGCGGTGAATACACTAACAGCCCTAGGAA
TTGACGCCGCAAGGATCAAGGCCTACACTCCAAGCTCAAGCTCTCCGACGGGCTTTGTACAGGTGAATGCCCCAAGCGCA
AAGGCTATTGTCAGTGGGCAAATGCCATATTATGGTACCCAAATTAGCCTGTCTGAAAAGGGTGACATCAACCTCTACCT
CTACCCTGAAGAGACACATTCATCAAGCAGCTCATCGAGCTCTGATACTTCAGCTTCTAACCCATCAGCTGGAGATACAA
CAGACTCAAGTAGTCATACCGAATTAGGTCAAAATGACTCTGCTGCAACACCGTAA

Domains

Predicted by InterproScan.

Protein kinase domain (Pkinase)

(12-232)

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380JRY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.042

94.295

0.613
  stkP/pknB Streptococcus salivarius strain HSISS4

62.245

93.185

0.58
  stkP Streptococcus pneumoniae TIGR4

59.302

81.775

0.485
  stkP Streptococcus pneumoniae D39

58.915

81.775

0.482
  stkP Streptococcus pneumoniae R6

58.915

81.775

0.482
  stkP Streptococcus pneumoniae Rx1

58.721

81.775

0.48