Detailed information NTG0876446

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RGQ10_RS09425
• Genome accession: NZ_CP133760
• Coordinates: 1909546..1910160 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain F37
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1904546..1915160

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGQ10_RS09395 (RGQ10_09395)	rpsT	1904849..1905115 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
RGQ10_RS09400 (RGQ10_09400)	holA	1905132..1906173 (-)	1042	Protein_1854	DNA polymerase III subunit delta	-
RGQ10_RS09405 (RGQ10_09405)	-	1906213..1906364 (-)	152	Protein_1855	hypothetical protein	-
RGQ10_RS09410 (RGQ10_09410)	-	1906405..1906539 (+)	135	WP_003152870.1	YqzM family protein	-
RGQ10_RS09415 (RGQ10_09415)	comEC	1906558..1908909 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RGQ10_RS09420 (RGQ10_09420)	-	1908910..1909479 (-)	570	WP_003152868.1	ComE operon protein 2	-
RGQ10_RS09425 (RGQ10_09425)	comEA	1909546..1910160 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
RGQ10_RS09430 (RGQ10_09430)	comER	1910219..1911040 (+)	822	WP_014418420.1	late competence protein ComER	-
RGQ10_RS09435 (RGQ10_09435)	-	1911109..1911846 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
RGQ10_RS09440 (RGQ10_09440)	rsfS	1911843..1912199 (-)	357	WP_007408260.1	ribosome silencing factor	-
RGQ10_RS09445 (RGQ10_09445)	yqeK	1912217..1912777 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RGQ10_RS09450 (RGQ10_09450)	-	1912767..1913336 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
RGQ10_RS09455 (RGQ10_09455)	yhbY	1913347..1913637 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
RGQ10_RS09460 (RGQ10_09460)	aroE	1913631..1914473 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=876446 RGQ10_RS09425 WP_014418419.1 1909546..1910160(-) (comEA) [Bacillus velezensis strain F37]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=876446 RGQ10_RS09425 WP_014418419.1 1909546..1910160(-) (comEA) [Bacillus velezensis strain F37]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363