Detailed information NTG0875585

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RFN60_RS12705
• Genome accession: NZ_CP133653
• Coordinates: 2480498..2481118 (-)
• Length: 206 a.a.
• NCBI ID: WP_024572262.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain CP38
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2475498..2486118

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RFN60_RS12675 (RFN60_12675)	rpsT	2475800..2476066 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
RFN60_RS12680 (RFN60_12680)	holA	2476081..2477124 (-)	1044	WP_015251674.1	DNA polymerase III subunit delta	-
RFN60_RS12685 (RFN60_12685)	-	2477164..2477313 (-)	150	WP_003229985.1	hypothetical protein	-
RFN60_RS12690 (RFN60_12690)	yqzM	2477354..2477488 (+)	135	WP_003229983.1	YqzM family protein	-
RFN60_RS12695 (RFN60_12695)	comEC	2477528..2479858 (-)	2331	WP_015251673.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RFN60_RS12700 (RFN60_12700)	comEB	2479862..2480431 (-)	570	WP_003229978.1	ComE operon protein 2	-
RFN60_RS12705 (RFN60_12705)	comEA	2480498..2481118 (-)	621	WP_024572262.1	competence protein ComEA	Machinery gene
RFN60_RS12710 (RFN60_12710)	comER	2481202..2482023 (+)	822	WP_024572261.1	late competence protein ComER	-
RFN60_RS12715 (RFN60_12715)	yqeM	2482089..2482832 (-)	744	WP_029946669.1	class I SAM-dependent methyltransferase	-
RFN60_RS12720 (RFN60_12720)	rsfS	2482829..2483185 (-)	357	WP_014480315.1	ribosome silencing factor	-
RFN60_RS12725 (RFN60_12725)	yqeK	2483203..2483763 (-)	561	WP_048655098.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RFN60_RS12730 (RFN60_12730)	nadD	2483753..2484322 (-)	570	WP_048655097.1	nicotinate-nucleotide adenylyltransferase	-
RFN60_RS12735 (RFN60_12735)	yhbY	2484334..2484624 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
RFN60_RS12740 (RFN60_12740)	aroE	2484618..2485460 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 206 a.a.
• Molecular weight: 21840.58 Da
• Isoelectric Point: 4.8354

>NTDB_id=875585 RFN60_RS12705 WP_024572262.1 2480498..2481118(-) (comEA) [Bacillus subtilis strain CP38]
MNWLKQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGGSVQSDGGKGALVNINTATLEELQGISG
VGPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 621 bp

>NTDB_id=875585 RFN60_RS12705 WP_024572262.1 2480498..2481118(-) (comEA) [Bacillus subtilis strain CP38]
ATGAATTGGTTGAAGCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGTGGTGGAGGGT
CTGTCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGG
GTGGGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAA
GGTTTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (143-204)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.029	100	0.99
comEA	Staphylococcus aureus MW2	36.364	100	0.388
comEA	Lactococcus lactis subsp. cremoris KW2	37.089	100	0.383
comEA	Staphylococcus aureus N315	35.455	100	0.379