Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RB214_RS14445 Genome accession   NZ_CP133569
Coordinates   3112932..3114068 (-) Length   378 a.a.
NCBI ID   WP_309167647.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. HL-AS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3107932..3119068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB214_RS14420 (RB214_14420) rsmE 3108480..3109214 (+) 735 WP_309167644.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RB214_RS14425 (RB214_14425) gshB 3109269..3110219 (+) 951 WP_309167645.1 glutathione synthase -
  RB214_RS14430 (RB214_14430) - 3110376..3110933 (+) 558 WP_309167646.1 YqgE/AlgH family protein -
  RB214_RS14435 (RB214_14435) ruvX 3110971..3111420 (+) 450 WP_002961947.1 Holliday junction resolvase RuvX -
  RB214_RS14440 (RB214_14440) - 3111560..3112888 (+) 1329 WP_375144327.1 IS4 family transposase -
  RB214_RS14445 (RB214_14445) pilU 3112932..3114068 (-) 1137 WP_309167647.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RB214_RS14450 (RB214_14450) pilT 3114078..3115118 (-) 1041 WP_309167648.1 type IV pilus twitching motility protein PilT Machinery gene
  RB214_RS14455 (RB214_14455) - 3115121..3115834 (+) 714 WP_182196817.1 YggS family pyridoxal phosphate-dependent enzyme -
  RB214_RS14460 (RB214_14460) proC 3115852..3116673 (+) 822 WP_002962567.1 pyrroline-5-carboxylate reductase -
  RB214_RS14465 (RB214_14465) - 3116700..3117233 (+) 534 WP_309167649.1 YggT family protein -
  RB214_RS14470 (RB214_14470) - 3117294..3117737 (+) 444 WP_309167650.1 DUF4426 domain-containing protein -
  RB214_RS14475 (RB214_14475) - 3117742..3118341 (+) 600 WP_309167651.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42025.98 Da        Isoelectric Point: 5.6401

>NTDB_id=874968 RB214_RS14445 WP_309167647.1 3112932..3114068(-) (pilU) [Pseudoalteromonas sp. HL-AS1]
MALSLNDFLLIMIEKKGSDLFVSSQLPVSAKINGELIALSDDRLTDEQSLELVESAMSEKQKAEFHTTKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDVSDLGLPSTLTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLVPTADGESRVAAIEILLNSPMVAELIKNGDIGGIKEAMAKSKEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKNRDQ

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=874968 RB214_RS14445 WP_309167647.1 3112932..3114068(-) (pilU) [Pseudoalteromonas sp. HL-AS1]
ATGGCTTTATCTTTAAACGACTTTTTACTCATCATGATTGAGAAAAAGGGTTCTGACCTATTTGTATCGAGTCAGTTACC
TGTAAGTGCAAAAATAAACGGCGAGCTAATAGCTCTCAGTGACGATAGACTTACTGATGAACAATCGCTTGAGTTAGTAG
AATCTGCAATGAGCGAAAAGCAAAAAGCAGAGTTTCATACAACGAAAGAATGTAACTTTGCGATTGCTACCGACGAAGGG
CGTTTTCGTATTTCTGCATTTTGGCAGCGCGATAGAGCAGGCATGGTTATTCGCCGTATTGTGACGCAAATTCCTGATGT
AAGTGATTTAGGTCTGCCATCTACGCTGACAGATGTAATTATGTCTAAACGCGGTCTTGTCTTGTTTGTAGGTGGTACAG
GTACGGGTAAGTCGACCTCACTAGCTGCACTTATTGGTTATCGAAACCGTAACCAACGAGGTCATATACTGACCATTGAA
GATCCGATTGAGTTTGTTCATGAGCATCGTAAAAGTATTATTACTCAGCGCGAAGTAGGGCTTGATACAGAAAGCTTTGA
GTCGGCACTTAAAAGCTCATTACGCCAAGCGCCAGATGTTATTTTGATTGGTGAAATTCGCTCGCAAGAAACCATGGAAT
ACGCACTAAGCTTTGCCGAAACCGGGCACCTATGTGTTGCAACACTACATGCTAATAACGCCAACCAAGCAATAGACCGT
ATCATGCATTTAGTTCCAAAAGAAAAACACGATAAGCTTAAATATGACTTAGCACTTAACTTACGCGCCATTGTTGCCCA
GCAATTAGTACCGACTGCTGATGGTGAAAGCCGTGTTGCAGCTATTGAAATATTGCTTAACTCACCAATGGTCGCTGAAC
TGATAAAAAATGGTGATATTGGCGGAATTAAAGAGGCAATGGCTAAGTCGAAAGAAATGGGCATGCAAACCTTTGACCAA
GCGTTATTTGAGTTGTACAGACAGCAGCGAATTAACTACGCTGATGCACTGCACCATGCTGACTCGCCAAATGATTTACG
CTTAATGATAAAACTGCGCAATAACGAACAACAAGGTGCGGGCTTTTTACAGGGCGTTACTATTGATGGCCTTGATGATA
AAAATAGGGATCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.067

99.206

0.566
  pilU Acinetobacter baylyi ADP1

56.233

99.735

0.561
  pilU Vibrio cholerae strain A1552

55.586

97.09

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.659

95.503

0.407